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Biolistic Transformation of Yeasts

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Yeast Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 53))

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Abstract

Biolistic transformation is a unique process in which DNA or RNA is introduced into cells on micron-sized particles. These microparticles are accelerated to supersonic speeds utilizing forces generated by a gunpowder discharge or cold gas explosion. This technique was first developed for transformation of plant cells (1). It has since been used successfully with bacteria (2), fungi (3), and mammalian cells, both in vitro (4) and in vivo (5).

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References

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© 1996 Humana Press Inc., Totowa, NJ

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Johnston, S.A., DeVit, M.J. (1996). Biolistic Transformation of Yeasts. In: Evans, I.H. (eds) Yeast Protocols. Methods in Molecular Biology™, vol 53. Humana Press. https://doi.org/10.1385/0-89603-319-8:147

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  • DOI: https://doi.org/10.1385/0-89603-319-8:147

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-319-1

  • Online ISBN: 978-1-59259-540-2

  • eBook Packages: Springer Protocols

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