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Plastid Transformation in Tomato

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Chloroplast Biotechnology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1132))

Abstract

Tomato (Solanum lycopersicum) is one of the most important vegetable crops and has long been an important model species in plant biology. Plastid biology in tomato is especially interesting due to the chloroplast-to-chromoplast conversion occurring during fruit ripening. Moreover, as tomato represents a major food crop with an edible fruit that can be eaten raw, the development of a plastid transformation protocol for tomato was of particular interest to plant biotechnology. Recent methodological improvements have made tomato plastid transformation more efficient and facilitated applications in metabolic engineering and molecular farming. This article describes the basic methods involved in the generation and analysis of tomato plants with transgenic chloroplast genomes and summarizes current applications of tomato plastid transformation.

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Acknowledgments

Work on plastid transformation in the authors’ laboratory is supported by the Max Planck Society and by grants from the Deutsche Forschungsgemeinschaft (DFG), the Bundesministerium für Bildung und Forschung (BMBF), and the European Union (Framework Programs 6 and 7).

Author information

Authors and Affiliations

  1. Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam-Golm, Germany

    Stephanie Ruf & Ralph Bock

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  1. Stephanie Ruf
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  2. Ralph Bock
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Editor information

Editors and Affiliations

  1. Rutgers University, Waksman Inst.of Microbiology, Piscataway, New Jersey, USA

    Pal Maliga

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Ruf, S., Bock, R. (2014). Plastid Transformation in Tomato. In: Maliga, P. (eds) Chloroplast Biotechnology. Methods in Molecular Biology, vol 1132. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-995-6_16

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  • DOI: https://doi.org/10.1007/978-1-62703-995-6_16

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-994-9

  • Online ISBN: 978-1-62703-995-6

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