Abstract
Nuclear resonance vibrational spectroscopy (NRVS) has been used by physicists for many years. However, it is still a relatively new technique for bioinorganic users. This technique yields a vibrational spectrum for a specific element, which can be easily interpreted. Furthermore, isotopic labeling allows for site-specific experiments. In this chapter, we discuss how to access specific beamlines, what kind of equipment is used in NRVS, and how the sample should be prepared and the data collected and analyzed.
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Acknowledgements
This work was funded by NIH grants GM-65440, EB-001962, and the DOE Office of Biological and Environmental Research. NRVS spectra were measured at APS Beamline 03ID, ESRF Beamline ID18, PETRA Beamline P01, and SPring-8 Beamlines 09XU, and BL19LXU. We also thank all our colleagues and collaborators for their assistances.
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Wang, H., Alp, E.E., Yoda, Y., Cramer, S.P. (2014). A Practical Guide for Nuclear Resonance Vibrational Spectroscopy (NRVS) of Biochemical Samples and Model Compounds. In: Fontecilla-Camps, J., Nicolet, Y. (eds) Metalloproteins. Methods in Molecular Biology, vol 1122. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-794-5_9
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DOI: https://doi.org/10.1007/978-1-62703-794-5_9
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Publisher Name: Humana Press, Totowa, NJ
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