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Genotyping Technologies: Application to Biotransformation Enzyme Genetic Polymorphism Screening

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Molecular Toxicology Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1105))

Abstract

Pharmacogenomics encompasses several major areas: the study of polymorphic variations to drug response and disease susceptibility, identification of the effects of drugs/xenobiotics at the genomic level, and genotype/phenotype associations. The most common type of human genetic variations is single-nucleotide polymorphisms (SNPs). Several novel approaches to detection of SNPs are currently available. The range of new methods includes modifications of several conventional techniques such as PCR, mass spectrometry, and sequencing as well as more innovative technologies such as fluorescence resonance energy transfer and microarrays. The application of each of these techniques is largely dependent on the number of SNPs to be screened and sample size. The current chapter presents an overview of the general concepts of a variety of genotyping technologies with an emphasis on the recently developed methodologies, including a comparison of the advantages, applicability, cost efficiency, and limitations of these methods.

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Author information

Authors and Affiliations

  1. Division of Hematology/Oncology, Department of Medicine, Hillman Cancer Center, 5117 Centre Avenue, Suite 1.32e, Pittsburgh, PA, 15213, USA

    Marjorie Romkes

  2. Center for Clinical Pharmacology, University of Pittsburgh, Pittsburgh, PA, USA

    Shama C. Buch

Authors
  1. Marjorie Romkes
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  2. Shama C. Buch
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Corresponding author

Correspondence to Marjorie Romkes .

Editor information

Editors and Affiliations

  1. Occupational Health, University of Pittsburgh Dept. Environmental &, Pittsburgh, Pennsylvania, USA

    Phouthone Keohavong

  2. Dept. of Pharmaceutical Sciences, Nova Southeastern University College of Pharmacy, Fort Lauderdale, Florida, USA

    Stephen G. Grant

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© 2014 Humana Press

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Romkes, M., Buch, S.C. (2014). Genotyping Technologies: Application to Biotransformation Enzyme Genetic Polymorphism Screening. In: Keohavong, P., Grant, S. (eds) Molecular Toxicology Protocols. Methods in Molecular Biology, vol 1105. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-739-6_8

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  • DOI: https://doi.org/10.1007/978-1-62703-739-6_8

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-738-9

  • Online ISBN: 978-1-62703-739-6

  • eBook Packages: Springer Protocols

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