Abstract
Functional analysis of G-proteins has been extensively carried out using their over-expressing lines and knockout mutants in plants. Since α subunit exists in an active or inactive form, overexpressing α subunit does not mean that G-protein signaling pathways are activated in the transgenic lines. Ectopic expression of the constitutively active form of the α subunit will magnify a role of G-protein signaling pathways in plant growth and development, and ultimately yield phenotypes. Here, we describe the method to study function of G-proteins in chloroplast development using the constitutively active form of the α subunit in Arabidopsis.
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References
Lapik YR, Kaufman LS (2003) The Arabidopsis cupin domain protein AtPirin1 interacts with the G protein a-subunit GPA1 and regulates seed germination and early seedling development. Plant Cell 15:1578–1590
Zhao J, Wang X (2004) Arabidopsis phospholipase Da 1 interacts with the heterotrimeric G-protein a-subunit through a motif analogous to the DRY motif in G-protein-coupled receptors. J Biol Chem 279:794–800
Warpeha KM, Upadhyay S, Yeh J, Adamiak J, Hawkins SI, Lapik YR, Anderson MB, Lee BS, Kaufman LS (2007) The GCR1, GPA1, PRN1, NF-Y signal chain mediates both blue light and abscisic acid responses in Arabidopsis. Plant Physiol 143:1590–1600
Huang J, Taylor JP, Chen J, Uhrig JF, Schnell DJ, Nakagawa T, Korth KL, Jones AM (2006) The plastid protein thylakoid formation 1 and the plasma membrane G-protein GPA1 interact in a novel sugar-signaling mechanism in Arabidopsis. Plant Cell 18:1226–1238
Wang Q, Sullivan RW, Kight A, Henry RL, Huang J, Jones AM, Korth KL (2004) Deletion of the chloroplast-localized thylakoid formation1 gene product in Arabidopsis leads to deficient thylakoid formation and variegated leaves. Plant Physiol 136:3594–3604
Mudgil Y, Uhrig JF, Zhou J, Temple B, Jiang K, Jones AM (2009) Arabidopsis N-MYC DOWNREGULATED-LIKE1, a positive regulator of auxin transport in a G protein-mediated pathway. Plant Cell 21:3591–3609
Zhang L, Wei Q, Wu W, Cheng Y, Hu G, Hu F, Sun Y, Zhu Y, Sakamoto W, Huang J (2009) Activation of the heterotrimeric G protein alpha-subunit GPA1 suppresses the ftsh-mediated inhibition of chloroplast development in Arabidopsis. Plant J 58:1041–1053
Chen M, Choi Y, Voytas DF, Rodermel SR (2000) Mutations in the Arabidopsis VAR2 locus cause leaf variegation due to the loss of a chloroplast FtsH protease. Plant J 22:303–313
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Wu, W., Huang, J. (2013). Functional Analysis of Heterotrimeric G Proteins in Chloroplast Development in Arabidopsis. In: Running, M. (eds) G Protein-Coupled Receptor Signaling in Plants. Methods in Molecular Biology, vol 1043. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-532-3_9
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DOI: https://doi.org/10.1007/978-1-62703-532-3_9
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-531-6
Online ISBN: 978-1-62703-532-3
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