Abstract
Myristoylation is a lipid modification conserved among eukaryotes and involves the addition of a 14-carbon myristoyl moiety to a glycine at the N-terminus of cargo proteins. Since not every protein with an N-terminal glycine is myristoylated, experimental verification is necessary to determine which proteins are indeed myristoylated. Here we describe an in vitro myristoylation assay for the Arabidopsis heterotrimeric G protein alpha subunit, GPA1, as well as the Arabidopsis SALT OVERLY SENSITIVE3. This method can be easily adopted to other proteins of interest.
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Feng, X., Shi, W., Wang, X., Running, M.P. (2013). In Vitro Myristoylation Assay of Arabidopsis Proteins. In: Running, M. (eds) G Protein-Coupled Receptor Signaling in Plants. Methods in Molecular Biology, vol 1043. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-532-3_14
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DOI: https://doi.org/10.1007/978-1-62703-532-3_14
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-531-6
Online ISBN: 978-1-62703-532-3
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