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Solid Phase Assay Measuring C4d Deposition to Determine Complement Fixation by HLA-Specific Antibodies

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Transplantation Immunology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1034))

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Abstract

The definition of HLA-specific antibodies in solid organ transplant patients is a necessary tool for recipient selection prior to transplantation and monitoring for rejection post transplant. Solid phase assays can detect both complement fixing and non-complement fixing HLA-specific antibodies. Here we describe a method for determining the presence of complement fixing HLA-specific antibodies using a sensitive solid phase assay.

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References

  1. Patel R, Terasaki PI (1969) Significance of the positive crossmatch test in kidney transplantation. N Engl J Med 280(14):735–739

    Article  PubMed  CAS  Google Scholar 

  2. Smith JD, Danskine AJ, Laylor RM, Rose ML, Yacoub MH (1993) The effect of panel reactive antibodies and the donor specific crossmatch on graft survival after heart and heart-lung transplantation. Transpl Immunol 1(1):60–65

    Article  PubMed  CAS  Google Scholar 

  3. Williams GM, Hume DM, Hudson RP Jr, Morris PJ, Kano K, Milgrom F (1968) “Hyperacute” renal-homograft rejection in man. N Engl J Med 279(12):611–618

    Article  PubMed  CAS  Google Scholar 

  4. McCloskey D, Festenstein H, Banner N et al (1989) The effect of HLA lymphocytotoxic antibody status and crossmatch result on cardiac transplant survival. Transplant Proc 21(1 Pt 1):804–806

    PubMed  CAS  Google Scholar 

  5. Smith JD, Hamour IM, Banner NR, Rose ML (2007) C4d fixing luminex binding antibodies – a new tool for prediction of graft failure after heart transplantation. Am J Transplant 7(122809–15 ):122809–122815

    Google Scholar 

  6. Gupta A, Iveson V, Varagunam M, Bodger S, Sinnott P, Thuraisingham RC (2008) Pretransplant donor-specific antibodies in cytotoxic negative crossmatch kidney transplants: are they relevant? Transplantation 85(8):1200–1204

    Article  PubMed  CAS  Google Scholar 

  7. Susal C, Ovens J, Mahmoud K et al (2011) No association of kidney graft loss with human leukocyte antigen antibodies detected exclusively by sensitive Luminex single-antigen testing: a Collaborative Transplant Study report. Transplantation 91(8):883–887

    Article  PubMed  Google Scholar 

  8. Wahrmann M, Exner M, Haidbauer B et al (2005) [C4d]FlowPRA® screening – a specific assay for selective detection of complement-­activating anti-HLA alloantibodies. Hum Immunol 66(5):526–534

    Article  PubMed  CAS  Google Scholar 

  9. Wahrmann M, Exner M, Schillinger M et al (2006) Pivotal role of complement-fixing HLA alloantibodies in presensitized kidney allograft recipients. Am J Transplant 6(5 Pt 1):1033–1041

    Article  PubMed  CAS  Google Scholar 

  10. Smith JD, Banner NR, Hamour IM et al (2011) De novo donor HLA-specific antibodies after heart transplantation are an independent predictor of poor patient survival. Am J Transplant 11(2):312–319

    Article  PubMed  CAS  Google Scholar 

  11. Hodges AM, Lyster H, McDermott A, et al (2012) Late antibody-mediated rejection after heart transplantation following the development of de novo donor-specific human leukocyte antigen antibody. Transplantation (Epub ahead of print)

    Google Scholar 

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Smith, J.D., Rose, M.L. (2013). Solid Phase Assay Measuring C4d Deposition to Determine Complement Fixation by HLA-Specific Antibodies. In: Zachary, A., Leffell, M. (eds) Transplantation Immunology. Methods in Molecular Biology, vol 1034. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-493-7_15

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  • DOI: https://doi.org/10.1007/978-1-62703-493-7_15

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-492-0

  • Online ISBN: 978-1-62703-493-7

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