Abstract
Kinetic profiling of drug binding to its target reveals important mechanistic parameters including drug–target residence time. In this chapter, we focus on global progress curve analysis as a convenient method for kinetic profiling. Detailed guidelines with pros and cons for various experimental designs and data analysis are provided. Kinetic profiling of Boceprevir and Telaprevir is illustrated.
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Acknowledgment
The authors gratefully acknowledge the technical assistance of Edward DiNunzio, the careful reading of the manuscript by Michael Kavana, and the enthusiastic managerial support from Christine Brideau.
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Zhang, R., Windsor, W.T. (2013). In Vitro Kinetic Profiling of Hepatitis C Virus NS3 Protease Inhibitors by Progress Curve Analysis. In: Gong, E. (eds) Antiviral Methods and Protocols. Methods in Molecular Biology, vol 1030. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-484-5_6
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DOI: https://doi.org/10.1007/978-1-62703-484-5_6
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