Abstract
Cyclic nucleotide phosphodiesterases regulate cellular levels of small molecule second messengers that control important biological processes in all kingdoms of life. Identifying and characterizing these enzymes is necessary for basic research and pharmaceutical applications. Here, we describe the use of thin layer chromatography to analyze cellular extracts or purified proteins for cyclic nucleotide phosphodiesterase activity.
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Purcell, E.B., Tamayo, R. (2013). Identification and Characterization of Cyclic Nucleotide Phosphodiesterases. In: Gehring, C. (eds) Cyclic Nucleotide Signaling in Plants. Methods in Molecular Biology, vol 1016. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-441-8_16
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DOI: https://doi.org/10.1007/978-1-62703-441-8_16
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-440-1
Online ISBN: 978-1-62703-441-8
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