Abstract
Defense signaling compounds and phytohormones play an essential role in the regulation of plant responses to various environmental abiotic and biotic stresses. Among the most severe stresses are herbivory, pathogen infection, and drought stress. The major hormones involved in the regulation of these responses are 12-oxo-phytodienoic acid (OPDA), the pro-hormone jasmonic acid (JA) and its biologically active isoleucine conjugate (JA-Ile), salicylic acid (SA), and abscisic acid (ABA). These signaling compounds are present and biologically active at very low concentrations from ng/g to μg/g dry weight. Accurate and sensitive quantification of these signals has made a significant contribution to the understanding of plant stress responses. Ultra-performance liquid chromatography (UPLC) coupled with a tandem quadrupole mass spectrometer (MS/MS) has become an essential technique for the analysis and quantification of these compounds.
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References
Glauser G, Dubugnon L, Mousavi SA, Rudaz S, Wolfender JL, Farmer EE (2009) Velocity estimates for signal propagation leading to systemic jasmonic acid accumulation in wounded Arabidopsis. J Biol Chem 284:34506–34513
Mueller MJ, Mene-Saffrane L, Grun C, Karg K, Farmer EE (2006) Oxylipin analysis methods. Plant J 45:472–489
Giannarelli S, Muscatello B, Bogani P, Spiriti MM, Buiatti M, Fuoco R (2010) Comparative determination of some phytohormones in wild-type and genetically modified plants by gas chromatography-mass spectrometry and high-performance liquid chromatography-tandem mass spectrometry. Anal Biochem 398:60–68
Pan X, Welti R, Wang X (2008) Simultaneous quantification of major phytohormones and related compounds in crude plant extracts by liquid chromatography–electrospray tandem mass spectrometry. Phytochemistry 69:1773–1781
Gundlach H, Muller MJ, Kutchan TM, Zenk MH (1992) Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures. Proc Natl Acad Sci USA 89:2389–2393
Kramell R, Schmidt J, Schneider G, Sembdner G, Schreiber K (1988) Synthesis of N-(jasmonoyl)amino acid conjugates. Tetrahedron 44:5791–5807
Gomez-Cadenas A, Pozo OJ, Garcia-Augustin P, Sancho JV (2002) Direct analysis of abscisic acid in crude plant extracts by liquid chromatography–electrospray/tandem mass spectrometry. Phytochem Anal 13:228–234
Pollard M, Ohlrogge J (1999) Testing models of fatty acid transfer and lipid synthesis in spinach leaf using in vivo oxygen-18 labeling. Plant Physiol 121:1217–1226
Acknowledgments
This work was supported by grants of the Graduiertenkolleg 1342 “Lipid signaling” and the SFB 567 of the Deutsche Forschungsgemeinschaft (DFG).
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Stingl, N., Krischke, M., Fekete, A., Mueller, M.J. (2013). Analysis of Defense Signals in Arabidopsis thaliana Leaves by Ultra-performance Liquid Chromatography/Tandem Mass Spectrometry: Jasmonates, Salicylic Acid, Abscisic Acid. In: Munnik, T., Heilmann, I. (eds) Plant Lipid Signaling Protocols. Methods in Molecular Biology, vol 1009. Humana, Totowa, NJ. https://doi.org/10.1007/978-1-62703-401-2_11
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DOI: https://doi.org/10.1007/978-1-62703-401-2_11
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