Abstract
Necrotic cell death (necrosis) occurs in many acute-onset diseases. However, our poor understanding of its mechanism has greatly limited medical interventions. Here we describe two methods to establish necrosis models in Drosophila. Our strategy is to overload calcium by expression of leaky cation channels.
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References
Yuan J (2009) Neuroprotective strategies targeting apoptotic and necrotic cell death for stroke. Apoptosis 14:469–477
Zong WX, Thompson CB (2006) Necrotic death as a cell fate. Genes Dev 20:1–15
Chalfie M, Wolinsky E (1990) The identification and suppression of inherited neurodegeneration in Caenorhabditis elegans. Nature 345:410–416
Xu K, Tavernarakis N, Driscoll M (2001) Necrotic cell death in C. elegans requires the function of calreticulin and regulators of Ca(2+) release from the endoplasmic reticulum. Neuron 31:957–971
Syntichaki P, Xu K, Driscoll M, Tavernarakis N (2002) Specific aspartyl and calpain proteases are required for neurodegeneration in C. elegans. Nature 419:939–944
Adams CM, Snyder PM, Price MP, Welsh MJ (1998) Protons activate brain Na+ channel 1 by inducing a conformational change that exposes a residue associated with neurodegeneration. J Biol Chem 273:30204–30207
Kohda K, Wang Y, Yuzaki M (2000) Mutation of a glutamate receptor motif reveals its role in gating and delta2 receptor channel properties. Nat Neurosci 3:315–322
Rubin GM, Spradling AC (1982) Genetic transformation of Drosophila with transposable element vectors. Science 218:348–353
Spradling AC, Rubin GM (1982) Transposition of cloned P elements into Drosophila germ line chromosomes. Science 218:341–347
Cardona A et al (2010) An integrated micro- and macroarchitectural analysis of the Drosophila brain by computer-assisted serial section electron microscopy. PLoS Biol 8(10):e1000502
Fan Y, Bergmann A (2009) The cleaved-Caspase-3 antibody is a marker of Caspase-9-like DRONC activity in Drosophila. Cell Death Differ 17:534–539
Wolff T (2010) Cryosectioning and immunocytochemistry of adult Drosophila eye sections. Cold Spring Harb Protoc 2010:pdb prot5370
Hutchins JB, Barger SW (1998) Why neurons die: cell death in the nervous system. Anat Rec 253:79–90
Giordano E, Rendina R, Peluso I, Furia M (2002) RNAi triggered by symmetrically transcribed transgenes in Drosophila melanogaster. Genetics 160:637–648
Hay BA, Wolff T, Rubin GM (1994) Expression of baculovirus P35 prevents cell death in Drosophila. Development 120:2121–2129
Steller H (2008) Regulation of apoptosis in Drosophila. Cell Death Differ 15:1132–1138
White K et al (1994) Genetic control of programmed cell death in Drosophila. Science 264:677–683
Chang YY, Neufeld TP (2009) An Atg1/Atg13 complex with multiple roles in TOR-mediated autophagy regulation. Mol Biol Cell 20:2004–2014
Mondal K et al (2007) Design and isolation of temperature-sensitive mutants of Gal4 in yeast and Drosophila. J Mol Biol 370:939–950
Eberl DF, Boekhoff-Falk G (2007) Development of Johnston’s organ in Drosophila. Int J Dev Biol 51:679–687
Loo DT, Rillema JR (1998) Measurement of cell death. Methods Cell Biol 57:251–264
Walsh GS, Orike N, Kaplan DR, Miller FD (2004) The invulnerability of adult neurons: a critical role for p73. J Neurosci 24:9638–9647
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Liu, K., Li, Y., Liu, L. (2013). Modeling Calcium-Overload Mediated Necrosis in Drosophila. In: McCall, K., Klein, C. (eds) Necrosis. Methods in Molecular Biology, vol 1004. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-383-1_15
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DOI: https://doi.org/10.1007/978-1-62703-383-1_15
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Publisher Name: Humana Press, Totowa, NJ
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