Abstract
Hematopoietic stem cells (HSCs) are capable of self-renewal and multi-lineage reconstitution of hematopoiesis in irradiated transplant recipient mice. As such, bone marrow transplantation (BMT) is a major assay commonly used to examine murine HSC activity. BMT traditionally involves injection of HSCs into lethally irradiated recipients via the tail vein, then subsequently analyzing donor engraftment. Here, we describe the methods for assaying HSC reconstitution in direct, competitive, and serial BMT.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Abramson S, Miller RG, Phillips RA (1977) The identification in adult bone marrow of pluripotent and restricted stem cells of the myeloid and lymphoid systems. J Exp Med 145:1567–1579
Lemischka IR, Raulet DH, Mulligan RC (1986) Developmental potential and dynamic behavior of hematopoietic stem cells. Cell 45:917–927
Geiger H, Van Zant G (2002) The aging of lympho-hematopoietic stem cells. Nat Immunol 3:329–333
de Haan G, Nijhof W, Van Zant G (1997) Mouse strain-dependent changes in frequency and proliferation of hematopoietic stem cells during aging: correlation between lifespan and cycling activity. Blood 89:1543–1550
Harrison DE, Astle CM, Delaittre JA (1978) Loss of proliferative capacity in immunohemopoietic stem cells caused by serial transplantation rather than aging. J Exp Med 147:1526–1531
Ford CE, Hamerton JL, Barnes DW, Loutit JF (1956) Cytological identification of radiation-chimaeras. Nature 177:452–454
McCulloch EA, Till JE (1960) The radiation sensitivity of normal mouse bone marrow cells, determined by quantitative marrow transplantation into irradiated mice. Radiat Res 13:115–125
Purton LE, Scadden DT (2007) Limiting factors in murine hematopoietic stem cell assays. Cell Stem Cell 1:263–270
Harrison DE (1980) Competitive repopulation: a new assay for long-term stem cell functional capacity. Blood 55:77–81
Rosendaal M, Hodgson GS, Bradle TR (1979) Organization of haemopoietic stem cells: the generation-age hypothesis. Cell Tissue Kinet 12:17–29
Purton LE, Dworkin S, Olsen GH, Walkley CR, Fabb SA, Collins SJ, Chambon P (2006) RAR{gamma} is critical for maintaining a balance between hematopoietic stem cell self-renewal and differentiation. J Exp Med 203:1283–1293
Ema H, Morita Y, Yamazaki S, Matsubara A, Seita J et al (2006) Adult mouse hematopoietic stem cells: purification and single-cell assays. Nat Protoc 1:2979–2987
Mauch P, Hellman S (1989) Loss of hematopoietic stem cell self-renewal after bone marrow transplantation. Blood 74:872–875
Yu H, Yuan Y, Shen H, Cheng T (2006) Hematopoietic stem cell exhaustion impacted by p18 INK4C and p21 Cip1/Waf1 in opposite manners. Blood 107:1200–1206
Kiel MJ, Yilmaz OH, Iwashita T, Yilmaz OH, Terhorst C, Morrison SJ (2005) SLAM family receptors distinguish hematopoietic stem and progenitor cells and reveal endothelial niches for stem cells. Cell 121:1109–1121
Acknowledgments
We thank Dr. Hongmei Shen for her critical review of this manuscript. This work was supported by the grants from the Ministry of Science and Technology of China (2011CB964800) and the National Science Foundation of China (81090410 and 30825017) to TC. TC was a recipient of the Scholar Award from the Leukemia & Lymphoma Society (1027–08).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Cheng, H., Liang, P.H., Cheng, T. (2013). Mouse Hematopoietic Stem Cell Transplantation. In: Turksen, K. (eds) Stem Cells and Aging. Methods in Molecular Biology, vol 976. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-317-6_3
Download citation
DOI: https://doi.org/10.1007/978-1-62703-317-6_3
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-316-9
Online ISBN: 978-1-62703-317-6
eBook Packages: Springer Protocols