Abstract
The ability of peripheral blood mononuclear cells (PBMCs) to act as a surrogate window into the presence and physiologic effects of pancreatic cancer is becoming increasingly apparent. In this chapter, we describe the techniques for isolation, lysis, RNA extraction, cDNA synthesis, and Q-RT PCR analysis of PBMCs as well as reasonable alternatives and the advantages and disadvantages of each. We further discuss the noteworthy considerations necessary for successful isolation and conversion of the high-quality PBMC RNA required to acquire interpretable and reproducible results for PBMC genetic expression analysis.
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References
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Acknowledgements
The authors on this work are supported, in part, by grants from the National Institutes of Health (EDRN UO1 CA111294, RO1 CA131944, and SPORE P50 CA127297).
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Baine, M.J., Mallya, K., Batra, S.K. (2013). Quantitative Real-Time PCR Expression Analysis of Peripheral Blood Mononuclear Cells in Pancreatic Cancer Patients. In: Su, G. (eds) Pancreatic Cancer. Methods in Molecular Biology, vol 980. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-287-2_8
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DOI: https://doi.org/10.1007/978-1-62703-287-2_8
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-286-5
Online ISBN: 978-1-62703-287-2
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