Abstract
Immunohistochemistry (IHC) is a valuable tool to visualize the distribution and localization of specific cellular components within morphologically preserved tissue sections or cell preparations. It combines the histologic morphology of tissues for detecting the actual antigen distribution, specificity of antibody–antigen interaction for optimal detection, and sensitivity of immunochemical methods for assessing the amount of antigen in tissues. It is routinely used clinically to diagnose type (benign or malignant), stage, and grade of cancer using specific tumor markers. The application of IHC ranges from disease diagnosis and prognosis to drug development and analysis of the pathobiological roles of various molecular players during disease development. Due to better availability of highly specific antibodies and optimal methodologies for performing immunohistochemical studies, IHC is being used at an expanding rate to understand pancreatic tumor biology as well as to study the fate of various molecular markers during the initiation, progression, and metastasis of pancreatic neoplasia. Herein, we describe the detailed protocol for IHC analyses of pancreatic intraepithelial neoplasia in tissues and fine needle aspirates from both human and mouse samples.
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Acknowledgements
The authors on this work are supported, in part, by grants from the National Institutes of Health (RO1 CA78590, EDRN UO1 CA111294, RO1 CA 131944, RO1 CA133774, SPORE P50 CA127297, and UO1 CA163120).
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Kaur, S., Shimizu, T., Baine, M.J., Kumar, S., Batra, S.K. (2013). Immunohistochemistry of Pancreatic Neoplasia. In: Su, G. (eds) Pancreatic Cancer. Methods in Molecular Biology, vol 980. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-287-2_3
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DOI: https://doi.org/10.1007/978-1-62703-287-2_3
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-286-5
Online ISBN: 978-1-62703-287-2
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