Abstract
MicroRNAs (miRNAs) play important roles in development, differentiation, homeostasis, and also in diseases such as lymphoma. This chapter describes methods to study the role of miRNAs in lymphoma. First, we describe a multiplex RT reaction followed by qPCR that can be used to determine differential expression of candidate miRNAs. Second, we provide a protocol for stable overexpression of miRNAs using lentiviral-based ectopic expression systems. Third, we describe a straightforward assay to determine whether the candidate miRNA may function as an oncogene or a tumor suppressor gene in lymphomagenesis.
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Acknowledgment
This work was supported by the Dutch Cancer Society (RUG 2009-4279 to A.v.d.B.).
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Kluiver, J., Slezak-Prochazka, I., van den Berg, A. (2013). Studying MicroRNAs in Lymphoma. In: Küppers, R. (eds) Lymphoma. Methods in Molecular Biology, vol 971. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-269-8_15
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DOI: https://doi.org/10.1007/978-1-62703-269-8_15
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