Abstract
Membrane-associated glycoproteins play critical roles in many biological processes and are often the therapeutic targets for drug discovery. Lectin affinity chromatography is one of the most widely used approaches for enrichment of glycoproteins at the protein level. Here, we describe a strategy for the characterization of membrane glycoproteins including membrane protein extraction, lectin affinity chromatography, protein digestion, and analysis by LC-MS/MS.
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Acknowledgment
This work was supported by the National Cancer Institute under grant R21 CA124441 (D.M.L.), and the National Institutes of Health Grant No. R01 GM49500 (D.M.L.).
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Liu, Y., He, J., Lubman, D.M. (2013). Characterization of Membrane-Associated Glycoproteins Using Lectin Affinity Chromatography and Mass Spectrometry. In: Kohler, J., Patrie, S. (eds) Mass Spectrometry of Glycoproteins. Methods in Molecular Biology, vol 951. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-146-2_6
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DOI: https://doi.org/10.1007/978-1-62703-146-2_6
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