Abstract
The mammalian hair follicle epithelial component contains various lineages of keratinocytes as well as their progenitor/stem cells. To characterize the subpopulations contained within this component and assess their functional capacity, the development of a feasible method to isolate them is greatly needed. In this chapter we describe a standard method by which a small subset of human follicular keratinocytes can be isolated for subsequent analysis. Detailed methods for enzymatic digestion of fresh human scalp, flow cytometry, cultivation of sorted cells and a colony forming assay are described.
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Acknowledgments
We thank other members of the Laboratory of Plastic Reconstructive Surgery in the University of Tokyo.
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Inoue, K., Yoshimura, K. (2013). Isolation and Characterization of Human Hair Follicle Epithelial Cells. In: Helgason, C., Miller, C. (eds) Basic Cell Culture Protocols. Methods in Molecular Biology, vol 946. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-128-8_26
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DOI: https://doi.org/10.1007/978-1-62703-128-8_26
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-127-1
Online ISBN: 978-1-62703-128-8
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