Abstract
Animal models of microbial diseases in humans are an essential component for determining fulfillment of Koch’s postulates and determining how the organism causes disease, host response(s), disease prevention, and treatment. In the case of Helicobacter pylori, establishing an animal model to fulfill Koch’s postulates initially proved so challenging that out of frustration a human volunteer undertook an experiment to become infected with H. pylori and to monitor disease progression in order to determine if it did cause gastritis. For the discovery of the organism and his fulfillment of Koch’s postulates he and a colleague were awarded the Nobel Prize in Medicine. After H. pylori was established as a gastric pathogen, it took several years before a model was developed in mice, opening the study of the organism and its pathogenicity to the general scientific community. However, while the model is widely utilized, there are a number of difficulties that can arise and need to be overcome. The purpose of this chapter is to raise awareness regarding the problems, and to offer reliable protocols for successfully establishing the H. pylori mouse model.
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References
Marshall BM, Armstrong JA, McGechie DB, Glancy RJ (1885) Attempt to fulfil Koch’s postulates for pyloric campylobacter. Med J Aust 142:436–439
IARC (1994) Schistosomes, liver flukes, and Helicobacter pylori. IARC Working Group on Evaluation of carcinogenic risks to humans. Lyon, 7–14 June 1994. IARC Monogr Eval Carcinogen Risks Hum. 61:1.
Cantorna MT, Balish E (1990) Inability of human clinical strains of Helicobacter pylori to colonize the alimentary tract of germ-free rodents. Can J Microbiol 36:237–241
Marchetti M, Arico B, Burroni D, Figura N, Rappouli R, Ghiara P (1995) Development of a mouse model of Helicobacter pylori infection that mimics human disease. Science 267:1655–1658
Lachman LB, Ozpolat B, Rao XM, Graham DY, Osato M (1997) Development of a murine model of Helicobacter pylori infection. Helicobacter 2:78–81
Krakowka S, Eaton KA, Rings DM, Morgan DR (1991) Gastritis induced by Helicobacter pylori in gnotobiotic piglets. Rev Infect Dis 8:S681–S685
Poutahidis T, Tsangaris T, Kanakoudis G, Vlemmas I, Iliadis N, Sofianou D (2001) Helicobacter pylori-induced gastritis in experimentally infected conventional piglets. Vet Pathol 38:667–678
Dubois A, Berg D, Incecik E, Fiala N, Heman Ackah L, Yang M, Wirth H, Perez-Perez GI, Blaser MJ (1999) Host specificity of Helicobacter pylori strains and host responses in experimentally challenged nonhuman primates. Gastroenterology 116:90–96
Fox JG, Perkins S, Yan L, Shen Z, Attardo L, Pappo J (1996) Local immune response in Helicobacter pylori-infected cats and identification of H. pylori in saliva, gastric fluid and faeces. Immunol 88:400–406
Karita M, Li Q, Cantero D, Okita K (1994) Establishment of a small animal model for human Helicobacter pylori infection in the germ free mouse. Am J Gastroenterol 89:208–213
Karita M, Li Q, Okita K (1993) Evaluation of new triple therapy for eradication of H. pylori infection in nude mouse model. Am J Gastroenterol 88:1366–1372
Radin JM, Eaton KA, Krakowa S, Lee A, Otto G, Fox JG (1990) Helicobacter pylori infection in gnotobiotic beagle dogs. Infect Immun 60:2606–2612
Yokita K, Kurebayashi Y, Takayama Y, Hayashi S, Isogai H, Isogai E, Imai K, Yabana T, Yachi A, Oguma K (1991) Colonization of Helicobacter pylori in the gastric tissue of Mongolian gerbils. Microbiol Immunol 35:475–480
Hirayama F, Takagi S, Kusuhara H, Iwao E, Yokoyama Y, Ikeda Y (1996) Induction of gastric ulcer and intestinal metaplasia in Mongolian gerbils infected with H. pylori. J Gastroenterol 31:755–757
Lee A, Fox JG, Otto G, Murphy J (1990) A small animal model of human Helicobacter pylori chronic gastritis. Gastroenterology 99:1315–1325
Paster BJ, Lee A, Fox JG, Dewhirst FE, Tordoff LA, Fraser GJ, O’Rourke JL, Ferrero R (1991) Phylogeny of Helicobacter felis sp. nov., Helicobacter mustelae, and related bacteria. Int J Syst Bacteriol 41:31–38
Enno A, O’Rourke JL, Howlett CR, Jack A, Dixon MF, Lee A (1995) MALToma-like lesions in the gastric mucosa after long term infection with Helicobacter felis. Am J Pathol 147:217–222
Fox JG, Cabot EB, Taylor NS, Laraway R (1988) Gastric colonization by Campylobacter pylori subsp. mustelae in ferrets. Infect Immun 56:2994–2996
Fox JG, Correa P, Taylor NS, Lee A, Otto G, Murphy JC, Rose R (1990) Helicobacter mustelae-associated gastritis in ferrets. An animal model of Helicobacter pylori gastritis in humans. Gastroenterology 99:352–361
Lee A, O’Rourke J, De Ungria J, Robertson B, Daskalopoulos G, Dixon MF (1997) A standardized mouse model of Helicobacter pylori infection: introducing the Sydney strain. Gastroenterology 112:1386–1397
Ferrero RL, Thiberge J-M, Huerre M, Labigne A (1998) Immune responses of specific-pathogen free mice to chronic Helicobacter pylori (strain SS1) infection. Infect Immun 66:1349–1355
Thompson LJ, Danon SJ, Wilson JE, O’Rourke JL, Salama NR, Falkow S, Mitchell H, Lee A (2004) Chronic Helicobacter pylori infection with the Sydney strain 1 and a newly identified mouse-adapted strain (Sydney strain 2000) in C57BL/6 and Balbc mice. Infect Immun 72:4668–4679
Israel DA, Salama N, Arnold CN, Moss SF, Ando T, Wirth HP, Tham KT, Camorlinga M, Blaser MJ, Falkow S, Peek RM Jr (2001) Helicobacter pylori strain-specific differences in genetic content, identified by microarray, influence host inflammatory responses. J Clin Invest 107:611–620
Fox JG, Wang TC, Rogers AB, Poutahidis T, Ge Z, Taylor NS, Dangler CA, Israel DA, Krishna U, Gaus K, Peek RM (2003) Host and microbial constituents influence Helicobacter pylori-induced cancer in a model of hypergastnemia. Gastroenterology 124:1879–1890
Rogers A, Houghton J (2009) Helicobacter-based mouse models of digestive system carcinogenesis. In: Kozlov SV (ed) Inflammation and cancer: methods in molecular biology, vol 511. pp 267–295
Bickley J, Owen RJ, Fraser AG, Pounder RE (1993) Evaluation of the polymerase chain reaction for detecting the urease C gene of Helicobacter pylori in gastric biopsy samples and dental plaque. J Med Mocrobiol 39:338–344
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Taylor, N.S., Fox, J.G. (2012). Animal Models of Helicobacter-Induced Disease: Methods to Successfully Infect the Mouse. In: Houghton, J. (eds) Helicobacter Species. Methods in Molecular Biology, vol 921. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-005-2_18
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DOI: https://doi.org/10.1007/978-1-62703-005-2_18
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