Abstract
Owing to the wide-ranging applications of noble metal nanoparticles in diverse areas of science and technology, different methods have been proposed for their synthesis. Here, we describe the methods for the intracellular biosynthesis of silver and gold nanoparticles using the bacterium Bacillus licheniformis KK2 and this same procedure can be followed for other bacteria as well. The biological synthesis of nanoparticles is highly eco-friendly and possesses distinct advantages such as enhanced stability, better control over the size, shape, and monodispersity of the nanoparticles, when compared with the more traditional physical and chemical methods which often involves the use of hazardous chemicals creating environmental concern.
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Acknowledgements
The authors wish to acknowledge the support of Dr. PusphaViswanathan, Professor, Cancer Institute (WIA), Chennai, who helped us with TEM analysis. Prof G. Sangiliyandi was supported by grants from Department of Science and Technology (DST), New Delhi (ProjectNo.SR/NM/NS-31/2010), India. Mr. Kalishwaralal was thankful to Council of Scientific and Industrial Research (CSIR), New Delhi for the award of Senior Research Fellowship (Reference No.9/10/2 (0003)/2k10-EMR-I (KK)).
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Sriram, M.I., Kalishwaralal, K., Gurunathan, S. (2012). Biosynthesis of Silver and Gold Nanoparticles Using Bacillus licheniformis . In: Soloviev, M. (eds) Nanoparticles in Biology and Medicine. Methods in Molecular Biology, vol 906. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-953-2_3
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DOI: https://doi.org/10.1007/978-1-61779-953-2_3
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