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The Application of Semiconductor Quantum Dots for Enhancing Peptide Desorption, Improving Peak Resolution and Sensitivity of Detection in Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry

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Nanoparticles in Biology and Medicine

Part of the book series: Methods in Molecular Biology ((MIMB,volume 906))

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Abstract

The interest in quantum dots (QD) and a number of reported life sciences applications increased dramatically over the last decade. The popularity of QDs stems from better photostability, higher extinction ­co-efficients, and unique optical properties such as superior light absorption. Here we report methods for improving matrix-assisted laser desorption/ionization desorption of crude tryptic protein digests by using CdSe/ZnS QDs. The addition of QDs to the matrix improves the signal-to-noise ratio, peak quality and increases the number of detected peptides and the overall sequence coverage.

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Authors and Affiliations

  1. School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey, UK

    Julian Bailes & Mikhail Soloviev

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  1. Julian Bailes
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  2. Mikhail Soloviev
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Correspondence to Mikhail Soloviev .

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  1. School of Biological Sciences, Royal Holloway University of London, Egham Hill, Egham, Surrey, TW20 0EX, United Kingdom

    Mikhail Soloviev

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Bailes, J., Soloviev, M. (2012). The Application of Semiconductor Quantum Dots for Enhancing Peptide Desorption, Improving Peak Resolution and Sensitivity of Detection in Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry. In: Soloviev, M. (eds) Nanoparticles in Biology and Medicine. Methods in Molecular Biology, vol 906. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-953-2_16

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  • DOI: https://doi.org/10.1007/978-1-61779-953-2_16

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-952-5

  • Online ISBN: 978-1-61779-953-2

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