Abstract
In recent years, in situ RNA hybridization technique has found a widespread application in developmental biology. This method has frequently been used to determine gene expression patterns, which is a first step toward understanding of a gene function. Here, we provide a reliable and sensitive method for in situ RNA hybridization on frozen sections of mouse embryo using digoxigenin-labeled RNA probes. This technique can be used to study gene expression patterns at all stages of odontogenesis.
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References
Nilaver G. (1986) In situ hybridization histochemistry as a supplement to immunohistochemistry. In Situ Hybridization in Brain. New York: Plenum Press.
Jin L. and Lloyd R. V. (1997) In situ hybridization: methods and applications. Journal of Clinical Laboratory Analysis 11, 2–9.
Buongiorno-Nardelli M. and Amaldi F. (1969) Autoradiographic detection of molecular hybrids between rRNA and DNA in tissue sections. Nature 225, 946–947.
John H. L., Birnstiel M. L., and Jones K.W. (1969) RNA-DNA hybrids at the cytological level. Nature 223, 912–913.
Chan V.T.-W. and McGee J.O’D. (1990) Non-radioactive probes: preparation, characterization, and detection. In Situ Hybridization: Principle and Practice (J. M. Polak & J. O’D. McGee eds.) Oxford: Oxford University Press.
Holtke HJ, Ankenbauer W, Muhlegger K, Rein R, Sagner G, Seibl R, Walter T. (1995) The digoxigenin (DIG) system for non-radioactive labelling and detection of nucleic acids – an overview. Cell Mol Biol 41(7), 883–905.
Herrington C. S., Burns J., Graham A. K., Evans M., and McGee J. (1989) Interphase cytogenetics using biotin and digoxigenin labelled probes I: relative sensitivity of both reporter molecules for detection of HPV16 in CaSki cells. J Clin Pathol 42, 601–606.
Schaeren-Wiemers N. and Gerfin-Moser A. (1993) A single protocol to detect transcripts of various types and expression level in neural tissue and cultured cells: in situ hybridization using digoxigenin-labelled cRNA probes. Histochemistry 100, 431–440.
Braissant O. and Wahli W. (1998) A simplified in situ hybridization protocol using non-radioactively labeled probes to detect abundant and rare mRNAs on tissue sections. Biochemica 1, 10–16.
Moter A, Göbel UB. (2000) Fluorescence in situ hybridisation (FISH) for direct visualization of microorganism. J Microbiol Meth 41, 85–112.
Nieto M. A., Patel K., and Wilkinson D. G. (1996) In situ hybridization analysis of chick embryos in whole mount and tissue sections. Methods Cell Biol. 51, 219–235.
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Kyrylkova, K., Kyryachenko, S., Kioussi, C., Leid, M. (2012). Determination of Gene Expression Patterns by In Situ Hybridization in Sections. In: Kioussi, C. (eds) Odontogenesis. Methods in Molecular Biology, vol 887. Humana Press. https://doi.org/10.1007/978-1-61779-860-3_3
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DOI: https://doi.org/10.1007/978-1-61779-860-3_3
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