Abstract
Whole-mount in situ hybridization (WISH) is a reliable and specific method to study three-dimensional patterns of gene expression. A labeled nucleic acid probe anneals to a complementary target sequence and is visualized and localized in the embryo. This chapter describes a sensitive method for WISH on mouse embryos using digoxigenin-labeled RNA probes. The technique can be used for the analysis of gene expression patterns during early stages of odontogenesis and in tooth explants.
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Kyryachenko, S., Kyrylkova, K., Leid, M., Kioussi, C. (2012). Determination of Gene Expression Patterns by Whole-Mount In Situ Hybridization. In: Kioussi, C. (eds) Odontogenesis. Methods in Molecular Biology, vol 887. Humana Press. https://doi.org/10.1007/978-1-61779-860-3_2
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DOI: https://doi.org/10.1007/978-1-61779-860-3_2
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