Abstract
Proteins are the focus of numerous xenotransplantation studies because they provide structure and function to the graft. Their presence, absence, or even a functional incompatibility among species can compromise the long-term functioning of the xenograft. In particular, many cell-surface and soluble proteins, such as cytokines and chemokines, are involved in triggering rejection. For this reason, the identification and characterization of key proteins for xenografting, of either pig or human origin, are very important. Understanding their role in the xenogeneic setting can set the bases for the development of genetic engineering approaches that prolong graft survival and ensure function. There are multiple ways of determining and attaining protein expression, as well as studying protein interactions. In this chapter, we describe some basic techniques that allow us to detect and characterize pig and human proteins in order to better understand the molecular bases of rejection and function of pig xenografts.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Costa C (2007) Immunobiology of xenograft rejection. In: Ulricker ON (ed) Transplantation immunology research trends. Nova, New York
Sommaggio R, Máñez R, Costa C (2009) TNF, pig CD86 and VCAM-1 identified as potential targets for intervention in xenotransplantation of pig chondrocytes. Cell Transplant 18:1381–1393
Costa C, Bell NK, Stabel TJ, Fodor WL (2004) Use of porcine tumor necrosis factor receptor 1-Ig fusion protein to prolong xenograft survival. Xenotransplantation 11:491–502
Seebach JD, Comrack C, Germana S et al (1997) HLA-Cw3 expression on porcine endothelial cells protects against xenogeneic cytotoxicity mediated by a subset of human NK cells. J Immunol 159:3655–3661
Otamiri M, Nilsson KG (1999) Analysis of human serum antibody-carbohydrate interaction using biosensor based on surface plasmon resonance. Int J Biol Macromol 26:263–268
Link C (1999) Selection of phage-displayed anti-guinea pig C5 or C5a antibodies and their application in xenotransplantation. Mol Immunol 36:1235–1247
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Uribe-Herranz, M., Costa, C. (2012). Basic Analyses of Proteins of Interest for Xenotransplantation. In: Costa, C., Máñez, R. (eds) Xenotransplantation. Methods in Molecular Biology, vol 885. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-845-0_3
Download citation
DOI: https://doi.org/10.1007/978-1-61779-845-0_3
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-844-3
Online ISBN: 978-1-61779-845-0
eBook Packages: Springer Protocols