Abstract
Xenotransplantation of hepatocytes is a future promise to treat liver diseases when there is a formal indication for transplantation. In this chapter, we describe techniques for hepatocyte xenotransplantation. The process was divided into three main steps: hepatocyte isolation, transplantation, and identification of donor cells in the recipient. Tips for each procedure are described at the notes section at the end of this chapter.
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References
Aleem KA, Parveen N, Habeeb MA et al (2006) Journey from hepatocyte transplantation to hepatic stem cells: a novel treatment strategy for liver diseases. Indian J Med Res 123:601–614
Habibullah CM, Syed IH, Qamar A et al (1992) Human fetal hepatocyte transplantation in patients with fulminant hepatic failure. Transplantation 58:951–952
Galvão FH, de Andrade Júnior DR, de Andrade DR et al (2006) Hepatocyte transplantation: state of the art. Hepatol Res 36:237–247
Fox IJ, Chowdhury JR (2004) Hepatocyte transplantation. Am J Transplant 4:7–13
Bonavita AG, Quaresma K, Cotta-de-Almeida V et al (2010) Hepatocyte xenotransplantation for treating liver disease. Xenotransplantation 17:181–187
Alves LA, Bonavita A, Quaresma K et al (2010) New strategies for acute liver failure: focus on xenotransplantation therapy. Cell Med B Cell Transplant 1:47–54
Soucek P, Zuber R, Anzenbacherová E et al (2001) Minipig cytochrome P450 3A, 2A and 2C enzymes have similar properties to human analogs. BMC Pharmacol 1:11
Kanazawa A, Platt JL (2000) Prospects for xenotransplantation of the liver. Semin Liver Dis 20:511–522
Berry MN, Friend DS (1969) High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study. J Cell Biol 43:506–520
Seglen PO (1976) Preparation of isolated rat liver cells. Meth Cell Biol 13:29–83
Fonseca PC, Nihei OK, Savino W et al (2006) Flow cytometry analysis of gap junction-mediated cell-cell communication: advantages and pitfalls. Cytometry A 69:487–493
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Lima-Quaresma, K.R.F., Bonavita, A.G., Cytrangulo, M.K., Pinto, M.A., Alves, L.A. (2012). Hepatocyte Xenotransplantation. In: Costa, C., Máñez, R. (eds) Xenotransplantation. Methods in Molecular Biology, vol 885. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-845-0_15
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DOI: https://doi.org/10.1007/978-1-61779-845-0_15
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Publisher Name: Humana Press, Totowa, NJ
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