Abstract
In this chapter, we describe the derivation and characterization of nine hIn this chapter, we describe the derivation and characterization of nine human embryonic stem cells (hESC) (VAL-3 to -11B) from different developmental embryo stages (inner cell mass from a blastocyst, morula, and blastomere from a 3-day embryo) under xeno-free conditions providing the necessary protocols and techniques to carry out their derivation, characterization, and propagation.
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Acknowledgments
This work was funded by the Regenerative Medicine Program, Conselleria de Sanidad, Generalitat Valenciana & Ministry of Science and Innovation, Spanish Government, PI Carlos Simon and FIS (PI08-1134) PI Diana Valbuena. Special acknowledgment are given to all members of Carlos Simón´s lab corresponding to the Valencia Node of the Stem Cell Bank as an essential part of their work, including Eva Gómez, Eva Sánchez, Diana Valbuena, Anabel Marqués-Marí, M Eugenia Póo, Verónica Ruiz, Cristóbal Aguilar, Marcia Riboldi, and José V Medrano.
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Galán, A., Simón, C. (2012). Human Embryonic Stem Cells Derived in Xeno-Free Conditions. In: Turksen, K. (eds) Human Embryonic Stem Cells Handbook. Methods in Molecular Biology, vol 873. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-794-1_2
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DOI: https://doi.org/10.1007/978-1-61779-794-1_2
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