Abstract
Chromatin immunoprecipitation (ChIP) followed by microarray-based (ChIP-Chip) or next-generation sequencing-based (ChIP-Seq) analysis has been established as a powerful and widely used method to investigate DNA–protein interactions relative to a genomic location in vivo. Here, we present a ChIP-Chip protocol, which utilizes an alternative, easier amplification protocol and when using high-quality ChIP-grade antibodies, will generate enough material for hybridization or sequencing with negligible enrichment bias due to amplification.
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Acknowledgments
We would like to thank Dr. Jenny Fischer for advice on random PCR amplifications. This work was supported by ENFIN, a Network of Excellence funded by the European Commission within its FP6 Programme, under the thematic area. “Life sciences, genomics and biotechnology for health,” contract number LSHG-CT-2005-518254, and also the Max Planck Society.
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Jung, M., Adjaye, J. (2012). Chromatin Immunoprecipitation-Based Analysis of Gene Regulatory Networks Operative in Human Embryonic Stem Cells. In: Turksen, K. (eds) Human Embryonic Stem Cells Handbook. Methods in Molecular Biology, vol 873. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-794-1_18
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DOI: https://doi.org/10.1007/978-1-61779-794-1_18
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-61779-794-1
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