Skip to main content
SpringerLink
Log in

Comparison of Neural Differentiation Potential of Human Pluripotent Stem Cell Lines Using a Quantitative Neural Differentiation Protocol

  • Protocol
  • First Online:
Human Embryonic Stem Cells Handbook

Part of the book series: Methods in Molecular Biology ((MIMB,volume 873))

Abstract

Neural differentiation of human embryonic (ES) and induced pluripotent (iPS) stem cell lines has been used for research in early human development, drug discovery, and cell replacement therapies. It is critical to establish generic differentiation protocols to compare the neural specification potential of each individually derived pluripotent stem cell line and identify the efficacious lines for research and therapeutic use. Here, we describe a reproducible and quantitative protocol to assess the neural progenitor (NP) generation of human pluripotent stem cell lines. This method includes a robust and well-defined neural inducing platform for Pax6+ neural rosette (neuroectodermal cells) generation, propagation, and subsequent differentiation into nestin+ NPs. A side-by-side comparison under common culture conditions among three human ES cell lines, TE03, TE06, and BG01V, and one iPS cell line, HD02, showed highly variable efficiency in their differentiation into NPs.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 59.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 79.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. Li XJ, Du ZW, Zarnowska ED, Pankratz M, Hansen LO, Pearce RA, Zhang SC (2005) Specification of motoneurons from human embryonic stem cells. Nat Biotechnol 23:215–221

    Article  PubMed  Google Scholar 

  2. Zhang SC (2006) Neural subtype specification from embryonic stem cells. Brain Pathol 16:132–142

    Article  PubMed  CAS  Google Scholar 

  3. Li XJ, Zhang SC (2006) In vitro differentiation of neural precursors from human embryonic stem cells. Methods Mol Biol 33:169–177

    Google Scholar 

  4. Ma W, Tavakoli T, Derby E, Serebryakova Y, Rao MS, Mattson MP (2008) Cell-extracellular matrix interactions regulate neural differentiation of human embryonic stem cells. BMC Dev Biol 8:90

    Article  PubMed  Google Scholar 

  5. Hu BY, Weick JP, Yu J, Ma LX, Zhang XQ, Thomson JA, Zhang SC (2010) Neural differentiation of human induced pluripotent stem cells follows developmental principles but with variable potency. Proc Natl Acad Sci USA 107:4335–4340

    Article  PubMed  CAS  Google Scholar 

  6. Hu BY, Du ZW, Zhang SC (2009) Differentiation of human oligodendrocytes from pluripotent stem cells. Nat Protoc 4:1614–1622

    Article  PubMed  CAS  Google Scholar 

  7. Hu BY, Zhang SC (2009) Differentiation of spinal motor neurons from pluripotent human stem cells. Nat Protoc 4:1295–1304

    Article  PubMed  CAS  Google Scholar 

  8. Zhang XQ, Zhang SC (2010) Differentiation of neural precursors and dopaminergic neurons from human embryonic stem cells. Methods Mol Biol 584:355–366

    Article  PubMed  CAS  Google Scholar 

  9. Swistowski A, Peng J, Liu Q, Mali P, Rao MS, Cheng L, Zeng X (2010) Efficient generation of functional dopaminergic neurons from human induced pluripotent stem cells under defined conditions. Stem Cells 28:1893–1904

    Article  PubMed  CAS  Google Scholar 

  10. Zeng H, Guo M, Martins-Taylor K, Wang X, Zhang Z, Park JW, Zhan S, Kronenberg MS, Lichtler A, Liu HX, Chen FP, Yue L, Li XJ, Xu RH (2010) Specification of region-specific neurons including forebrain glutamatergic neurons from human induced pluripotent stem cells. PLoS One 5:e11853

    Article  PubMed  Google Scholar 

  11. Tavakoli T, Derby E, Serebryakova Y, Rao MS, Mattson MP, Ma W (2009) Self-renewal and differentiation capabilities are variable between human embryonic stem cell lines I3, I6 and BG01V. BMC Cell Biol 10:44

    Article  PubMed  Google Scholar 

  12. Huan Q, Gao X, Wang Y, Shen Y, Ma W, Chen ZJ (2010) Comparative evaluation of human embryonic stem cell lines derived from zygotes with normal and abnormal pronuclei. Dev Dyn 239:425–438

    Article  PubMed  CAS  Google Scholar 

  13. Gao X, Yan J, Shen Y, Li M, Ma S, Wang J, Huan Q, Huang S, Ma W, Chen ZJ (2010) Human fetal trophonema matrix and uterine endometrium support better human embryonic stem cell growth and neural differentiation than mouse embryonic fibroblasts. Cell Reprogram 12:295–303

    Article  PubMed  CAS  Google Scholar 

  14. Amit M, Itskovitz-Eldor J (2002) Derivation and spontaneous differentiation of human embryonic stem cells. J Anat 200(Pt 3):225–232

    Article  PubMed  Google Scholar 

  15. Zeng X, Miura T, Luo Y, Bhattacharya B, Condie B, Chen J, Ginis I, Lyons I, Mejido J, Puri RK, Rao MS, Freed WJ (2004) Properties of pluripotent human embryonic stem cells BG01 and BG02. Stem Cells 22:292–312

    Article  PubMed  CAS  Google Scholar 

  16. Zeng X, Chen J, Liu Y, Luo Y, Schulz TC, Robins AJ, Rao MS, Freed WJ (2004) BG01V: a variant human embryonic stem cell line which exhibits rapid growth after passaging and reliable dopaminergic differentiation. Restor Neurol Neurosci 22:421–428

    PubMed  CAS  Google Scholar 

Download references

Acknowledgment

The authors wish to thank Yevgeniya Serebryakova and Eric Derby for their technical supports.

Author information

Authors and Affiliations

  1. Stem Cell Center, American Type Culture Collection, Manassas, VA, USA

    Dezhong Yin & Tahereh Tavakoli

  2. Renji Stem Cell Research Center, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China

    Wei-Qiang Gao

  3. Ectycell, Genopole Campus 1, 5 rue Henri Desbruères, F-91030, EVRY Cedex, France

    Wu Ma

Authors
  1. Dezhong Yin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tahereh Tavakoli
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wei-Qiang Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wu Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Wu Ma .

Editor information

Editors and Affiliations

  1. Sprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Smyth Road 501, Ottawa, K1Y 8L6, Canada

    Kursad Turksen

Rights and permissions

Reprints and permissions

Copyright information

© 2012 Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Yin, D., Tavakoli, T., Gao, WQ., Ma, W. (2012). Comparison of Neural Differentiation Potential of Human Pluripotent Stem Cell Lines Using a Quantitative Neural Differentiation Protocol. In: Turksen, K. (eds) Human Embryonic Stem Cells Handbook. Methods in Molecular Biology, vol 873. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-794-1_16

Download citation

  • DOI: https://doi.org/10.1007/978-1-61779-794-1_16

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-793-4

  • Online ISBN: 978-1-61779-794-1

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation