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An Introduction to Planar Chromatography and Its Application to Natural Products Isolation

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Natural Products Isolation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 864))

Abstract

Thin-layer chromatography (TLC) is an easy, inexpensive, rapid, and the most widely used method for the analysis and isolation of small organic natural and synthetic products. It also has use in the biological evaluation of organic compounds, particularly in the areas of antimicrobial and antioxidant metabolites and for the evaluation of acetylcholinesterase inhibitors which have utility in the treatment of Alzheimer’s disease. The ease and inexpensiveness of use of this technique, coupled with the ability to rapidly develop separation and bioassay protocols will ensure that TLC will be used for some considerable time alongside conventional instrumental methods. This chapter deals with the basic principles of TLC and describes methods for the analysis and isolation of natural products. Examples of methods for isolation of several classes of natural product are detailed and protocols for TLC bioassays are given.

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Author information

Authors and Affiliations

  1. Department of Pharmaceutical and Biological Chemistry, The School of Pharmacy, UCL School of Pharmacy, London, UK

    Simon Gibbons

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  1. Simon Gibbons
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Correspondence to Simon Gibbons .

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Editors and Affiliations

  1. School of Applied Sciences, Department of Pharmacy, University of Wolverhampton, Wulfruna Street, Wolverhampton, WV1 1LY, United Kingdom

    Satyajit D. Sarker

  2. , Leicester School of Pharmacy, De Montfort University, The Gateway, Leicester, LE1 9BH, United Kingdom

    Lutfun Nahar

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Gibbons, S. (2012). An Introduction to Planar Chromatography and Its Application to Natural Products Isolation. In: Sarker, S., Nahar, L. (eds) Natural Products Isolation. Methods in Molecular Biology, vol 864. Humana Press. https://doi.org/10.1007/978-1-61779-624-1_6

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  • DOI: https://doi.org/10.1007/978-1-61779-624-1_6

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-623-4

  • Online ISBN: 978-1-61779-624-1

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