Abstract
Central neuropathic pain is associated with many disease states including multiple sclerosis, stroke, and spinal cord injury, and is poorly managed. One type of central neuropathic pain that is particularly debilitating and challenging to treat is pain that occurs below the level of injury (below-level pain). The study of central neuropathic pain is commonly performed using animal models of stroke and spinal cord injury. Most of the spinal cord injury models currently being used were originally developed to model the gross physiological impact of clinical spinal cord injury. In contrast, the T13/L1 dorsal root avulsion model of spinal cord injury described here was developed specifically for the study of central pain, and as such, was developed to minimize confounding complications, such as paralysis, urinary tract infections, and autotomy. As such, this model induces robust and reliable hindpaw mechanical allodynia. Two versions of the model are described. The first is optimal for testing systemically administered pharmacological manipulations. The second was developed to accommodate intrathecal application of pharmacological manipulations. This model provides an additional means by which to investigate central pain states associated with spinal cord injury, including below-level pain. Finally, a brief discussion of at-level pain measurement is described as it has been suggested in the literature that the mechanisms underlying below- and at-level pain are different.
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References
Baastrup, C., and Finnerup, N. B. (2008) Pharmacological management of neuropathic pain following spinal cord injury. CNS Drugs 22, 455–475.
Siddall, P. J. (2009) Management of neuropathic pain following spinal cord injury: now and in the future. Spinal Cord 47, 352–359.
Siddall, P. J., McClelland, J. M., Rutkowski, S. B., and Cousins, M. J. (2003) A longitudinal study of the prevalence and characteristics of pain in the first 5 years following spinal cord injury. Pain 103, 249–257.
Jensen, M. P., Kuehn, C. M., Amtmann, D., and Cardenas, D. D. (2007) Symptom burden in persons with spinal cord injury. Arch. Phys. Med. Rehabil. 88, 638–645.
Summers, J. D., Rapoff, M. A., Varghese, G., Porter, K., and Palmer, R. E. (1991) Psychosocial factors in chronic spinal cord injury pain. Pain 47, 183–189.
Westgren, N., and Levi, R. (1998) Quality of life and traumatic spinal cord injury. Arch. Phys. Med. Rehabil. 79, 1433–1439.
Christensen, M. D., Everhart, A. W., Pickelman, J. T., and Hulsebosch, C. E. (1996) Mechanical and thermal allodynia in chronic central pain following spinal cord injury. Pain 68, 97–107.
Christensen, M. D., and Hulsebosch, C. E. (1997) Chronic central pain after spinal cord injury. J. Neurotrauma 14, 517–537.
Hulsebosch, C. E., Xu, G. Y., Perez-Polo, J. R., Westlund, K. N., Taylor, C. P., and McAdoo, D. J. (2000) Rodent model of chronic central pain after spinal cord contusion injury and effects of gabapentin. J. Neurotrauma 17, 1205–1217.
Lampert, A., Hains, B. C., and Waxman, S. G. (2006) Upregulation of persistent and ramp sodium current in dorsal horn neurons after spinal cord injury. Exp. Brain Res. 174, 660–666.
Tan, A. M., Stamboulian, S., Chang, Y. W., Zhao, P., Hains, A. B., Waxman, S. G., and Hains, B. C. (2008) Neuropathic pain memory is maintained by Rac1-regulated dendritic spine remodeling after spinal cord injury. J. Neurosci. 28, 13173–13183.
Edgar, R. E., Best, L. G., Quail, P. A., and Obert, A. D. (1993) Computer-assisted DREZ microcoagulation: posttraumatic spinal deafferentation pain. J. Spinal Disord 6, 48–56.
Itoh, T., Hirota, K., Hisano, T., Namba, T., and Fukuda, K. (2004) The volatile anesthetics halothane and isoflurane differentially modulate proinflammatory cytokine-induced p38 mitogen-activated protein kinase activation. J. Anesth. 18, 203–209.
Kudo, M., Aono, M., Lee, Y., Massey, G., Pearlstein, R. D., and Warner, D. S. (2001) Effects of volatile anesthetics on N-methyl-D-aspartate excitotoxicity in primary rat neuronal-glial cultures. Anesthesiology 95, 756–765.
Martin, D. C., Plagenhoef, M., Abraham, J., Dennison, R. L., and Aronstam, R. S. (1995) Volatile anesthetics and glutamate activation of N-methyl-D-aspartate receptors. Biochem. Pharmacol. 49, 809–817.
Roughan, J. V., and Laming, P. R. (1998) Large slow potential shifts occur during halothane anaesthesia in gerbils. J. Comp. Physiol. 182, 839–848.
Toda, N., Toda, H., and Hatano, Y. (2008) Anesthetic modulation of immune reactions mediated by nitric oxide. J. Anesth. 22, 155–162.
Wentlandt, K., Samoilova, M., Carlen, P. L., and El Beheiry, H. (2006) General anesthetics inhibit gap junction communication in cultured organotypic hippocampal slices. Anesth. Analg. 102, 1692–1698.
Wise-Faberowski, L., Pearlstein, R. D., and Warner, D. S. (2006) NMDA-induced apoptosis in mixed neuronal/glial cortical cell cultures: the effects of isoflurane and dizocilpine. J. Neurosurg. Anesthesiol. 18, 240–246.
Wieseler, J., Ellis, A. L., McFadden, A., Brown, K., Starnes, C., Maier, S. F., Watkins, L. R., and Falci, S. (2010) Below level central pain induced by discrete dorsal spinal cord injury. J Neurotrauma 27, 1697–1707.
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Sources of financial and material support: Craig Hospital and DA024044.
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Wieseler, J., Ellis, A., Maier, S.F., Watkins, L.R., Falci, S. (2012). Unilateral T13 and L1 Dorsal Root Avulsion: Methods for a Novel Model of Central Neuropathic Pain. In: Luo, Z. (eds) Pain Research. Methods in Molecular Biology, vol 851. Humana Press. https://doi.org/10.1007/978-1-61779-561-9_12
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DOI: https://doi.org/10.1007/978-1-61779-561-9_12
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