Abstract
Orofacial pain remains an understudied area in pain research given that most attention has been focused on the spinal system. In this chapter, animal models of neuropathic and inflammatory orofacial pain are presented. Four different types of pain behavior tests are then described for assessing evoked and spontaneous pain behavior in addition to conditional reward behavior. The use of a combination of different pain models and behavior assessments is needed to aid in understanding the mechanisms contributing to orofacial pain in humans for developing effective therapy.
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References
Fried, K., Bongenhielm, U., Boissonade, F. M., and Robinson, P. P. (2001) Nerve injury-induced pain in the trigeminal system, Neuroscientist 7, 155–165.
Kitt, C. A., Gruber, K., Davis, M., Woolf, C. J., and Levine, J. D. (2000) Trigeminal neuralgia: opportunities for research and treatment, Pain 85, 3–7.
Vos, B. P., Strassman, A. M., and Maciewicz, R. J. (1994) Behavioral evidence of trigeminal neuropathic pain following chronic constriction injury to the rat’s infraorbital nerve, J Neurosci 14, 2708–2723.
Vos, B. P., and Strassman, A. M. (1995) Fos expression in the medullary dorsal horn of the rat after chronic constriction injury to the infraorbital nerve, J Comp Neurol 357, 362–375.
Imamura, Y., Kawamoto, H., and Nakanishi, O. (1997) Characterization of heat-hyperalgesia in an experimental trigeminal neuropathy in rats, Exp Brain Res 116, 97–103.
Ohara, P. T., Vit, J. P., Bhargava, A., and Jasmin, L. (2008) Evidence for a role of connexin 43 in trigeminal pain using RNA interference in vivo, J Neurophysiol 100, 3064–3073.
Vit, J. P., Ohara, P. T., Bhargava, A., Kelley, K., and Jasmin, L. (2008) Silencing the Kir4.1 potassium channel subunit in satellite glial cells of the rat trigeminal ganglion results in pain-like behavior in the absence of nerve injury, J Neurosci 28, 4161–4171.
Dubuisson, D., and Dennis, S. G. (1977) The formalin test: a quantitative study of the analgesic effects of morphine, meperidine, and brain stem stimulation in rats and cats, Pain 4, 161–174.
Dallel, R., Raboisson, P., Clavelou, P., Saade, M., and Woda, A. (1995) Evidence for a peripheral origin of the tonic nociceptive response to subcutaneous formalin, Pain 61, 11–16.
Raboisson, P., and Dallel, R. (2004) The orofacial formalin test, Neurosci Biobehav Rev 28, 219–226.
Mogil, J. S. (2009) Animal models of pain: progress and challenges, Nat Rev Neurosci 10, 283–294.
Nagamine, K., Ozaki, N., Shinoda, M., Asai, H., Nishiguchi, H., Mitsudo, K., Tohnai, I., Ueda, M., and Sugiura, Y. (2006) Mechanical allodynia and thermal hyperalgesia induced by experimental squamous cell carcinoma of the lower gingiva in rats, J Pain 7, 659–670.
Ono, K., Harano, N., Nagahata, S., Seta, Y., Tsujisawa, T., Inenaga, K., and Nakanishi, O. (2009) Behavioral characteristics and c-Fos expression in the medullary dorsal horn in a rat model for orofacial cancer pain, Eur J Pain 13, 373–379.
Holland, G. R. (1996) Experimental trigeminal nerve injury, Crit Rev Oral Biol Med 7, 237–258.
Chichorro, J. G., Zampronio, A. R., Souza, G. E., and Rae, G. A. (2006) Orofacial cold hyperalgesia due to infraorbital nerve constriction injury in rats: reversal by endothelin receptor antagonists but not non-steroidal anti-inflammatory drugs, Pain 123, 64–74.
Morgan, J. R., and Gebhart, G. F. (2008) Characterization of a model of chronic orofacial hyperalgesia in the rat: contribution of NA(V) 1.8, J Pain 9, 522–531.
Okada-Ogawa, A., Suzuki, I., Sessle, B. J., Chiang, C. Y., Salter, M. W., Dostrovsky, J. O., Tsuboi, Y., Kondo, M., Kitagawa, J., Kobayashi, A., Noma, N., Imamura, Y., and Iwata, K. (2009) Astroglia in medullary dorsal horn (trigeminal spinal subnucleus caudalis) are involved in trigeminal neuropathic pain mechanisms, J Neurosci 29, 11161–11171.
Neubert, J. K., Widmer, C. G., Malphurs, W., Rossi, H. L., Vierck, C. J., Jr., and Caudle, R. M. (2005) Use of a novel thermal operant behavioral assay for characterization of orofacial pain sensitivity, Pain 116, 386–395.
Rossi, H. L., Vierck, C. J., Jr., Caudle, R. M., and Neubert, J. K. (2006) Characterization of cold sensitivity and thermal preference using an operant orofacial assay, Mol Pain 2, 37.
Kernisant, M., Gear, R. W., Jasmin, L., Vit, J. P., and Ohara, P. T. (2008) Chronic constriction injury of the infraorbital nerve in the rat using modified syringe needle, J Neurosci Methods 172, 43–47.
Bennett, G. J., and Xie, Y. K. (1988) A peripheral mononeuropathy in rat that produces disorders of pain sensation like those seen in man, Pain 33, 87–107.
Clavelou, P., Pajot, J., Dallel, R., and Raboisson, P. (1989) Application of the formalin test to the study of orofacial pain in the rat, Neurosci Lett 103, 349–353.
Clavelou, P., Dallel, R., Orliaguet, T., Woda, A., and Raboisson, P. (1995) The orofacial formalin test in rats: effects of different formalin concentrations, Pain 62, 295–301.
Eisenberg, E., Vos, B. P., and Strassman, A. M. (1996) The peripheral antinociceptive effect of morphine in a rat model of facial pain, Neuroscience 72, 519–525.
Chichorro, J. G., Lorenzetti, B. B., and Zampronio, A. R. (2004) Involvement of bradykinin, cytokines, sympathetic amines and prostaglandins in formalin-induced orofacial nociception in rats, Br J Pharmacol 141, 1175–1184.
Jasmin, L., Vit, J. P., Bhargava, A., and Ohara, P. T. (2010) Can satellite glial cells be therapeutic targets for pain control?, Neuron Glia Biol 6, 63–71.
Vit, J. P., Ohara, P. T., Sundberg, C., Rubi, B., Maechler, P., Liu, C., Puntel, M., Lowenstein, P., Castro, M., and Jasmin, L. (2009) Adenovector GAD65 gene delivery into the rat trigeminal ganglion produces orofacial analgesia, Mol Pain 5, 42.
Davis, J. D., and Campbell, C. S. (1973) Peripheral control of meal size in the rat. Effect of sham feeding on meal size and drinking rate, J Comp Physiol Psychol 83, 379–387.
Vit, J. P., Jasmin, L., Bhargava, A., and Ohara, P. T. (2006) Satellite glial cells in the trigeminal ganglion as a determinant of orofacial neuropathic pain, Neuron Glia Biol 2, 247–257.
Vos, B. P., Hans, G., and Adriaensen, H. (1998) Behavioral assessment of facial pain in rats: face grooming patterns after painful and non-painful sensory disturbances in the territory of the rat’s infraorbital nerve, Pain 76, 173–178.
Pelissier, T., Pajot, J., and Dallel, R. (2002) The orofacial capsaicin test in rats: effects of different capsaicin concentrations and morphine, Pain 96, 81–87.
Acknowledgments
This work is supported by NIH grants NS051336 and NS061241.
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Kaan, T.K.Y., Ohara, P.T., Jasmin, L. (2012). Orofacial Pain Models and Behavior Assessment. In: Luo, Z. (eds) Pain Research. Methods in Molecular Biology, vol 851. Humana Press. https://doi.org/10.1007/978-1-61779-561-9_11
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DOI: https://doi.org/10.1007/978-1-61779-561-9_11
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