Abstract
Detailed methods for the preparation of optic nerve head (ONH) astrocytes from human donor eyes and retinal astrocytes from rat eyes are described. Included is the immunopanning method used for ONH astrocyte isolation as well as cell characterization. The isolation of purified retinal astrocytes is outlined as a method applicable to rodent and other mammalian retinas.
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Acknowledgments
This work was supported in part by NIH grant EY06416 (M.R. Hernandez) and unrestricted funds from Research to Prevent Blindness to the Department of Ophthalmology of Northwestern University.
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Lukas, T.J., Wang, A.L. (2012). Isolation and Culture of Astrocytes from the Retina and Optic Nerve. In: Milner, R. (eds) Astrocytes. Methods in Molecular Biology, vol 814. Humana Press. https://doi.org/10.1007/978-1-61779-452-0_8
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DOI: https://doi.org/10.1007/978-1-61779-452-0_8
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