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Histomorphometry in Rodents

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Bone Research Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 816))

Abstract

Bone histomorphometry remains an important tool to study the pathophysiology of bone disease and the cellular mechanism by which treatments work. Here, we review the methods for embedding, sectioning, staining, and analysis of bone sections in rodents.

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References

  1. Parfitt, A. M., Drezner, M. K., Glorieux, F. H., Kanis, J. A., Malluche, H., Meunier, P. J., Ott, S. M., and Recker, R. R. (1987) Bone histomorphometry: standardization of nomenclature, symbols, and units. Report of the ASBMR Histomorphometry Nomenclature Committee. J. Bone Miner. Res. 2, 595–610.

    CAS  Google Scholar 

  2. Erben, R. G. (1997) Embedding of bone samples in methylmethacrylate: An improved method suitable for bone histomorphometry, histochemistry, and immunohistochemistry. J. Histochem. Cytochem. 45, 307–313.

    Article  PubMed  CAS  Google Scholar 

  3. Schenk, R. K., Olah, A. J., and Herrmann, W. (1984) Preparation of calcified tissues for light microscopy. In Methods of calcified tissue preparation (Dickson, G.R. Ed.), pp. 1–56. Elsevier, Amsterdam.

    Google Scholar 

  4. Reim, N. S., Breig, B., Stahr, K., Eberle, J., Hoeflich, A., Wolf, E., and Erben, R. G. (2008) Cortical bone loss in androgen-deficient aged male rats is mainly caused by increased endocortical bone remodeling. J. Bone Miner. Res. 23, 694–704.

    Article  PubMed  CAS  Google Scholar 

  5. Baron, R., Vignery, A., Neff, L., Silverglate, A., and Santa Maria, A. (1983) Processing of ­undecalcified bone specimens for bone histomorphometry. In Bone Histomorphometry: Techniques and Interpretation (Recker, R.R. Ed.), pp. 13–35. CRC Press, Boca Raton, FL.

    Google Scholar 

  6. Erben, R. G. (2003) Bone Labeling Techniques. In Handbook of Histology Methods for Bone and Cartilage (An, Y.H. and Martin, K.L. Eds.), pp. 99–117. Humana Press Inc, Totowa, NJ, USA.

    Chapter  Google Scholar 

  7. Erben, R. G., Scutt, A. M., Miao, D. S., Kollenkirchen, U., and Haberey, M. (1997) Short-term treatment of rats with high dose 1,25-dihydroxyvitamin D3 stimulates bone formation and increases the number of osteoblast precursor cells in bone marrow. Endocrinology 138, 4629–4635.

    Article  PubMed  CAS  Google Scholar 

  8. Frost, H. M. (1983) Bone histomorphometry: choice of marking agent and labeling schedule. In Bone Histomorphometry: Techniques and Interpretation (Recker, R.R. Ed.), pp. 37–52. CRC Press, Boca Raton, FL.

    Google Scholar 

  9. Frost, H. M. (1983) Bone histomorphometry: analysis of trabecular bone dynamics. In Bone Histomorphometry: Techniques and Interpretation (Recker, R.R. Ed.), pp. 109–131. CRC Press, Boca Raton, FL.

    Google Scholar 

  10. Erben, R. G. (1996) Trabecular and endocortical bone surfaces in the rat: Modeling or remodeling? Anat. Rec. 246, 39–46.

    Article  PubMed  CAS  Google Scholar 

  11. Reim, N. S., Breig, B., Stahr, K., Eberle, J., Hoeflich, A., Wolf, E., and Erben, R. G. (2008) Cortical bone loss in androgen-deficient aged male rats is mainly caused by increased endocortical bone remodeling. J. Bone Miner. Res. 23, 694–704.

    Article  PubMed  CAS  Google Scholar 

  12. Ibbotson, K. J., Orcutt, C. M., D’Souza, S. M., Paddock, C. L., Arthur, J. A., Jankowsky, M. L., and Boyce, R. W. (1992) Contrasting effects of parathyroid hormone and insulin-like growth factor I in an aged ovariectomized rat model of postmenopausal osteoporosis. J. Bone. Miner. Res., 7, 425–432.

    Article  PubMed  CAS  Google Scholar 

  13. Lauritzen, D. B., Balena, R., Shea, M., Seedor, J. G., Markatos, A., Le, H. M., Toolan, B. C., Myers, E. R., Rodan, G. A., and Hayes, W. C. (1993) Effects of combined prostaglandin and alendronate treatment on the histomorphometry and biomechanical properties of bone in ovariectomized rats. J. Bone Miner. Res. 8, 871–879.

    Article  PubMed  CAS  Google Scholar 

  14. Uzawa, T., Hori, M., Ejiri, S. and Ozawa, H. (1995) Comparison of the effects of intermittent and continuous administration of human parathyroid hormone(1–34) on rat bone. Bone 16, 477–484.

    PubMed  CAS  Google Scholar 

  15. Bentolila, V., Boyce, T. M., Fyhrie, D. P., Drumb, R., Skerry, T. M., and Schaffler, M. B. (1998) Intracortical remodeling in adult rat long bones after fatigue loading. Bone 23, 275–281.

    Article  PubMed  CAS  Google Scholar 

  16. Weber, K., Kaschig, C., and Erben, R. G. (2004) 1alpha-Hydroxyvitamin D2 and 1alpha-hydroxyvitamin D3 have anabolic effects on cortical bone, but induce intracortical remodeling at toxic doses in ovariectomized rats. Bone 35, 704–710.

    Article  PubMed  CAS  Google Scholar 

  17. Erben, R. G., Eberle, J., Stahr, K., and Goldberg, M. (2000) Androgen deficiency induces high turnover osteopenia in aged male rats: a sequential histomorphometric study. J. Bone Miner. Res. 15, 1085–1098.

    Article  PubMed  CAS  Google Scholar 

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Correspondence to Reinhold G. Erben .

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Erben, R.G., Glösmann, M. (2012). Histomorphometry in Rodents. In: Helfrich, M., Ralston, S. (eds) Bone Research Protocols. Methods in Molecular Biology, vol 816. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-415-5_19

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  • DOI: https://doi.org/10.1007/978-1-61779-415-5_19

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-414-8

  • Online ISBN: 978-1-61779-415-5

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