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Effects of Temperature Generated from the Holmium: YAG Laser on Human Osteoblasts in Monolayer Tissue Culture

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Human Cell Culture Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 806))

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Abstract

With the use of lasers for ablation purposes in spinal surgery, the tissue temperature increases above the boiling point of water, leading to tissue ablation by vaporisation. Due to the thermal environment engendered by the use of lasers, there is concern about the safety of the surrounding important structures, such as dura mater, dorsal root ganglia, and nerve roots.

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Correspondence to Moustafa I. Hafez .

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Hafez, M.I., Sandison, A., Coombs, R.R.H., McCarthy, I.D., Hafez, AS.M. (2012). Effects of Temperature Generated from the Holmium: YAG Laser on Human Osteoblasts in Monolayer Tissue Culture. In: Mitry, R., Hughes, R. (eds) Human Cell Culture Protocols. Methods in Molecular Biology, vol 806. Humana Press. https://doi.org/10.1007/978-1-61779-367-7_24

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  • DOI: https://doi.org/10.1007/978-1-61779-367-7_24

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-366-0

  • Online ISBN: 978-1-61779-367-7

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