Abstract
Methods to detect and characterize cellular protein myristoylation and palmitoylation are invaluable in cell biology, immunology, and virology. Recently, we developed ω-alkynyl fatty acid probes for monitoring myristoylation and palmitoylation of cellular proteins. This article describes a biochemical procedure for metabolic labeling of cells with ω-alkynyl fatty acids and click chemistry.
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Hannoush, R.N. (2012). Profiling Cellular Myristoylation and Palmitoylation Using ω-Alkynyl Fatty Acids. In: Zanders, E. (eds) Chemical Genomics and Proteomics. Methods in Molecular Biology, vol 800. Humana Press. https://doi.org/10.1007/978-1-61779-349-3_7
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DOI: https://doi.org/10.1007/978-1-61779-349-3_7
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