Abstract
Mass spectrometry (MS) provides quantitative information toward accurate mass identification of unknown molecules and has become a powerful and widely used technique for bioanalysis. In this chapter, we describe the fabrication and MS analysis steps for a high sensitivity matrix-free laser desorption/ionization mass spectrometry (LDI-MS) target substrate termed nanofilament silicon (nSi). Unlike other nanostructured porous silicon surfaces, nSi possesses an open pore morphology with associated benefits including efficient transport of sample into the nanopores and effective removal of solvent from the surface. The utility of nSi targets for LDI-MS analysis lies primarily in the analysis of small molecules, which are typically less than 5,000 Da with a detection sensitivity in the attomole range.
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References
Wei, J., Buriak, J.M., Siuzdak, G. (1999) Desorption-ionization mass spectrometry on porous silicon. Nature 399, 243–246.
Trauger, S.A., Go, E.P., Shen, Z.X., et al. (2004) High sensitivity and analyte capture with desorption/ionization mass spectrometry on silylated porous silicon. Anal Chem 76, 4484–4489.
Nayak, R., Knapp, D.R. (2007) Effects of thin-film structural parameters on laser desorption/ionization from porous alumina. Anal Chem 79, 4950–4956.
Kang, M.J., Pyun, J.C., Lee, J.C., et al. (2005) Nanowire-assisted laser desorption and ionization mass spectrometry for quantitative analysis of small molecules. Rapid Commun Mass Spectrom 19, 3166–3170.
Finkel, N.H., Prevo, B.G., Velev, O.D., et al. (2005) Ordered silicon nanocavity arrays in surface-assisted desorption/ionization mass spectrometry. Anal Chem 77, 1088–1095.
Li, X., Bohn, P.W. (2000) Metal-assisted chemical etching in HF/H2O2 produces porous silicon. Appl Phys Lett 77, 2572–2574.
Tsao, C.W., Kumar, P., Liu, J., et al.(2008) Dynamic electrowetting on nanofilament silicon for matrix-free laser desorption/ionization mass spectrometry. Anal Chem 80, 2973–2981.
Shen, Z.X., Thomas, J.J., Averbuj, C., et al. (2001) Porous silicon as a versatile platform for laser desorption/ionization mass spectrometry. Anal Chem 73, 612–619.
Kruse, R.A., Li, X.L., Bohn, P.W., et al. (2001) Experimental factors controlling analyte ion generation in laser desorption/ionization mass spectrometry on porous silicon. Anal Chem 73, 3639–3645.
Alimpiev, S., Grechnikov, A., Sunner, J., et al. (2008) On the role of defects and surface chemistry for surface-assisted laser desorption ionization from silicon. J Chem Phys 128, 19 pages.
Cruz, S., Honig-dOrville, A., Muller, J. (2005) Fabrication and optimization of porous silicon substrates for diffusion membrane applications. J Electrochem Soc 152, C418–C424.
Acknowledgments
The authors would like to thank the National Science Council, Taiwan (NSC 97-2218-E-008-010-MY2) and the National Institutes of Health (R01GM072512) for their financial support.
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Tsao, CW., DeVoe, D.L. (2011). Nanofilament Silicon for Matrix-Free Laser Desorption/Ionization Mass Spectrometry. In: Toms, S., Weil, R. (eds) Nanoproteomics. Methods in Molecular Biology, vol 790. Humana Press. https://doi.org/10.1007/978-1-61779-319-6_14
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DOI: https://doi.org/10.1007/978-1-61779-319-6_14
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