Abstract
To expedite the development of personalized medicine, new and reliable biomarkers are required to facilitate early diagnosis, to determine prognosis, predict response or resistance to different therapies, and to monitor disease progression or recurrence. Human body fluids, such as blood, present a promising resource for biomarker discovery, in every sense. Microspot immunoassays allow the simultaneous quantification of multiple analytes from a minute amount of samples in a single measurement. The experimental design of microspot immunoassays is based on antibody pairs recognizing different epitopes of the analyte. The first antibody is used to capture the analyte from the complex sample, and the second antibody is used for detection. As with traditional enzyme-linked immunosorbent assays, highly reliable and reproducible results are obtained.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Biomarkers Definitions Working Group (2001) Biomarkers and surrogate endpoints: preferred definitions and conceptual framework, Clin Pharmacol Ther 69, 89–95.
American Diabetes Association (2010) Diagnosis and classification of diabetes mellitus, Diabetes Care 33 Suppl 1, S62-69.
Donegan, W. L. (1992) Prognostic factors. Stage and receptor status in breast cancer, Cancer 70, 1755–1764.
Donnelly, J. G. (2004) Pharmacogenetics in cancer chemotherapy: balancing toxicity and response, Ther Drug Monit 26, 231–235.
Dunn, L., and Demichele, A. (2009) Genomic predictors of outcome and treatment response in breast cancer, Mol Diagn Ther 13, 73–90.
Duffy, M. J., and Crown, J. (2008) A personalized approach to cancer treatment: how biomarkers can help, Clin Chem 54, 1770–1779.
Goldhirsch, A., Ingle, J. N., Gelber, R. D., Coates, A. S., Thurlimann, B., and Senn, H. J. (2009) Thresholds for therapies: highlights of the St Gallen International Expert Consensus on the primary therapy of early breast cancer 2009, Ann Oncol 20, 1319–1329.
Lilja, H., Ulmert, D., and Vickers, A. J. (2008) Prostate-specific antigen and prostate cancer: prediction, detection and monitoring, Nat Rev Cancer 8, 268–278.
Molina, R., Barak, V., van Dalen, A., Duffy, M. J., Einarsson, R., Gion, M., Goike, H., Lamerz, R., Nap, M., Soletormos, G., and Stieber, P. (2005) Tumor markers in breast cancer- European Group on Tumor Markers recommendations, Tumour Biol 26, 281–293.
Hanash, S. M., Pitteri, S. J., and Faca, V. M. (2008) Mining the plasma proteome for cancer biomarkers, Nature 452, 571–579.
Rai, A. J. (2007) Biomarkers in translational research: focus on discovery, development and translation of protein biomarkers to clinical immunoassays, Expert Rev Mol Diagn 7, 545–553.
Jöcker, A., Sonntag, J., Henjes, F., Gotschel, F., Tresch, A., Beissbarth, T., Wiemann, S., and Korf, U. (2010) QuantProReloaded: quantitative analysis of microspot immunoassays, Bioinformatics 26, 2480–2481.
Sweep, F. C. G. J., Thomas, C. M. G., and Schmitt, M. (2006) Analytical aspects of biomarker immunoassays in cancer research, in Biomarkers in breast cancer: Molecular diagnostics for predicting and monitoring therapeutic effect (Gasparini, G., and Hayes, D. F., Eds.), pp 17–30, Humana Press.
Gonzalez, R. M., Seurynck-Servoss, S. L., Crowley, S. A., Brown, M., Omenn, G. S., Hayes, D. F., and Zangar, R. C. (2008) Development and validation of sandwich ELISA microarrays with minimal assay interference, J Proteome Res 7, 2406–2414.
Jelkmann, W. (2001) Pitfalls in the measurement of circulating vascular endothelial growth factor, Clin Chem 47, 617–623.
Rai, A. J., Gelfand, C. A., Haywood, B. C., Warunek, D. J., Yi, J., Schuchard, M. D., Mehigh, R. J., Cockrill, S. L., Scott, G. B., Tammen, H., Schulz-Knappe, P., Speicher, D. W., Vitzthum, F., Haab, B. B., Siest, G., and Chan, D. W. (2005) HUPO Plasma Proteome Project specimen collection and handling: towards the standardization of parameters for plasma proteome samples, Proteomics 5, 3262–3277.
Acknowledgments
This work was supported by the German Federal Ministry for Education and Science in the framework of the Program for Medical Genome Research (grants 01GS0890 and 01GS0864), the Program for Medical Systems Biology (grant 0315396B), as well as the Helmholtz Systems Biology Initiative (SBCancer). Thanks go also to Liz Segal for her help with proofreading.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2011 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Sonntag, J., Mannsperger, H., Jöcker, A., Korf, U. (2011). Microspot Immunoassay-Based Analysis of Plasma Protein Profiles for Biomarker Discovery Strategies. In: Korf, U. (eds) Protein Microarrays. Methods in Molecular Biology, vol 785. Humana Press. https://doi.org/10.1007/978-1-61779-286-1_16
Download citation
DOI: https://doi.org/10.1007/978-1-61779-286-1_16
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-61779-285-4
Online ISBN: 978-1-61779-286-1
eBook Packages: Springer Protocols