Abstract
PARP-1 is the most abundantly expressed member of a family of proteins that catalyze the transfer of ADP-ribose units from NAD+ to target proteins. Numerous studies on PARP-1 have revealed its critical roles in regulating various molecular and cellular processes including DNA damage detection and repair, chromatin modification.
In this chapter, we describe various in vitro methods to investigate the function of PARP-1 in the regulation of chromatin structure using chromatin templates.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Kim MY, Zhang T, Kraus WL (2005) Poly(ADP-ribosyl)ation by PARP-1: ‘PAR-laying’ NAD+ into a nuclear signal. Genes Dev 19:1951–1967
Kotova E, Jarnik M, Tulin AV (2010) Uncoupling of the trans-activation and trans-repression functions of PARP1 protein. Proc Natl Acad Sci U S A 107:6406–6411
Kraus WL (2008) Transcriptional control by PARP-1: chromatin modulation, enhancer-binding, coregulation, and insulation. Curr Opin Cell Biol 20:294–302
Kraus WL, Lis JT (2003) PARP goes transcription. Cell 113:677–683
Ziegler M, Oei SL (2001) A cellular survival switch: poly(ADP-ribosyl)ation stimulates DNA repair and silences transcription. Bioessays 23:543–548
Kim MY, Mauro S, Gevry N, Lis JT, Kraus WL (2004) NAD + -dependent modulation of chromatin structure and transcription by nucleosome binding properties of PARP-1. Cell 119:803–814
Krishnakumar R, Gamble MJ, Frizzell KM, Berrocal JG, Kininis M, Kraus WL (2008) Reciprocal binding of PARP-1 and histone H1 at promoters specifies transcriptional outcomes. Science 319:819–821
Tulin A, Spradling A (2003) Chromatin loosening by poly(ADP)-ribose polymerase (PARP) at Drosophila puff loci. Science 299:560–562
Fyodorov DV, Kadonaga JT (2003) Chromatin assembly in vitro with purified recombinant ACF and NAP-1. Methods Enzymol 371:499–515
Ito T, Bulger M, Kobayashi R, Kadonaga JT (1996) Drosophila NAP-1 is a core histone chaperone that functions in ATP-facilitated assembly of regularly spaced nucleosomal arrays. Mol Cell Biol 16:3112–3124
Ito T, Levenstein ME, Fyodorov DV, Kutach AK, Kobayashi R, Kadonaga JT (1999) ACF consists of two subunits, Acf1 and ISWI, that function cooperatively in the ATP-dependent catalysis of chromatin assembly. Genes Dev 13:1529–1539
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2011 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Kim, M.Y. (2011). Regulation of Chromatin Structure by PARP-1. In: Tulin, A. (eds) Poly(ADP-ribose) Polymerase. Methods in Molecular Biology, vol 780. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-270-0_14
Download citation
DOI: https://doi.org/10.1007/978-1-61779-270-0_14
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-269-4
Online ISBN: 978-1-61779-270-0
eBook Packages: Springer Protocols