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Molecular Biology and Genetic Engineering in Nitrogen Fixation

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Nitrogen Fixation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 766))

Abstract

Biological nitrogen fixation is a complex and tightly regulated process limited to a group of prokaryotic species known as diazotrophs. Among well-studied diazotrophs, Azotobacter vinelandii is the best studied for its convenience of aerobic growth, its high levels of nitrogenase expression, and its genetic tractability. This chapter includes protocols and strategies in the molecular biology and genetic engineering of A. vinelandii that have been used as valuable tools for advancing studies on the biosynthesis, mechanism, and regulation of nitrogen fixation.

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Authors and Affiliations

  1. Department of Chemistry, Wake Forest University, Wiston-Salem, NC, 27109, USA

    Patricia C. Dos Santos

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  1. Patricia C. Dos Santos
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Correspondence to Patricia C. Dos Santos .

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Editors and Affiliations

  1. , Dept Molecular Biology & Biochemistry, University of California, Irvine, Irvine, 92697, California, USA

    Markus W. Ribbe

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Dos Santos, P.C. (2011). Molecular Biology and Genetic Engineering in Nitrogen Fixation. In: Ribbe, M. (eds) Nitrogen Fixation. Methods in Molecular Biology, vol 766. Humana Press. https://doi.org/10.1007/978-1-61779-194-9_6

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  • DOI: https://doi.org/10.1007/978-1-61779-194-9_6

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-193-2

  • Online ISBN: 978-1-61779-194-9

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