Abstract
mRNA extraction and subsequent RT-polymerase chain reaction (PCR)-based expression analysis from laser-microdissected material is by now a well-established and reproducible method. Most routinely stored tissue samples are preserved as formalin-fixed, paraffin-embedded materials. While this allows for a convenient storage and stable preservation of nucleic acids, deparaffinization before staining for laser microdissection may result in a significant loss of mRNA quality and consequently of PCR sensitivity. We describe a method of isolating anatomic compartments from non-deparaffinized, formalin-fixed, and paraffin-embedded tissues by laser-assisted microdissection which allows for a highly efficient mRNA retrieval.
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Jonigk, D., Modde, F., Bockmeyer, C.L., Becker, J.U., Lehmann, U. (2011). Optimized RNA Extraction from Non-deparaffinized, Laser-Microdissected Material. In: Murray, G. (eds) Laser Capture Microdissection. Methods in Molecular Biology, vol 755. Humana Press. https://doi.org/10.1007/978-1-61779-163-5_5
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DOI: https://doi.org/10.1007/978-1-61779-163-5_5
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Online ISBN: 978-1-61779-163-5
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