Abstract
Laser-capture microdissection (LCM) is an excellent tool to selectively obtain target tissue or cells. The endolymphatic sac (ES) is part of the inner ear, and a large part of the ES is located in the temporal bone. The rat ES is conventionally harvested using stereomicroscopy. In this method, contamination is unavoidable because of its size and location; therefore, additional checks, such as in situ hybridization, are necessary to confirm the cellular localization, and quantitative analysis is difficult in the ES. We have shown a selective epithelial tissue method using LCM to obtain RNA without contamination from ES epithelial tissue.
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Acknowledgments
This work was supported in part by Grants-in-Aid for Scientific Research (#13671783 and #16591711 to N.M., #16791003 and #791208 to T.M.).
An outline of this work was published in ref. 9 and is reproduced with permission from Elsevier Science.
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Akiyama, K., Miyashita, T., Matsubara, A., Mori, N. (2011). Specific RNA Collection from the Rat Endolymphatic Sac by Laser-Capture Microdissection (LCM): LCM of a Very Small Organ Surrounded by Bony Tissues. In: Murray, G. (eds) Laser Capture Microdissection. Methods in Molecular Biology, vol 755. Humana Press. https://doi.org/10.1007/978-1-61779-163-5_37
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DOI: https://doi.org/10.1007/978-1-61779-163-5_37
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