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Rapid Cloning and Validation of MicroRNA Shuttle Vectors: A Practical Guide

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RNA Interference Techniques

Part of the book series: Neuromethods ((NM,volume 58))

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Abstract

MicroRNAs (miRNAs) have emerged as important modulators of eukaryotic gene expression through a process called RNA interference (RNAi). Over the last several years, a large amount of work has focused on understanding how miRNAs are expressed and processed to a biologically functional form. This knowledge has enabled the development of RNAi as a molecular tool for investigating basic biological questions or as a therapeutic technique. Artificial miRNA shuttle vectors can be engineered to mimic natural miRNAs and subsequently used to suppress any gene of interest. Here, we describe a simple method to build and functionally validate artificial miRNA shuttles.

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Author information

Authors and Affiliations

  1. Center for Gene Therapy, The Research Institute at Nationwide Children’s Hospital, Columbus, OH, USA

    Scott Q. Harper

  2. Department of Pediatrics and Molecular, Cellular, and Developmental Biology Graduate Program, The Ohio State University College of Medicine, Columbus, OH, USA

    Scott Q. Harper

Authors
  1. Ryan L. Boudreau
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  2. Sara E. Garwick-Coppens
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  3. Jian Liu
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  4. Lindsay M. Wallace
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  5. Scott Q. Harper
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Corresponding author

Correspondence to Scott Q. Harper .

Editor information

Editors and Affiliations

  1. Research Institute, Center for Gene Therapy, Nationwide Children’s Hospital, Children's Drive 700, Columbus, 43205, Ohio, USA

    Scott Q. Harper

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© 2011 Springer Science+Business Media, LLC

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Boudreau, R.L., Garwick-Coppens, S.E., Liu, J., Wallace, L.M., Harper, S.Q. (2011). Rapid Cloning and Validation of MicroRNA Shuttle Vectors: A Practical Guide. In: Harper, S. (eds) RNA Interference Techniques. Neuromethods, vol 58. Humana Press. https://doi.org/10.1007/978-1-61779-114-7_2

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  • DOI: https://doi.org/10.1007/978-1-61779-114-7_2

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-113-0

  • Online ISBN: 978-1-61779-114-7

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