Abstract
The basics of cell culture are now relatively common, though it was not always so. The pioneers of cell culture would envy our simple access to manufactured plastics, media and equipment for such studies. The prerequisites for cell culture are a well lit and suitably ventilated laboratory with a laminar flow hood (Class II), CO2 incubator, benchtop centrifuge, microscope, plasticware (flasks and plates) and a supply of media with or without serum supplements. Not only can all of this be ordered easily over the internet, but large numbers of well-characterised cell lines are available from libraries maintained to a very high standard allowing the researcher to commence experiments rapidly and economically. Attention to safety and disposal is important, and maintenance of equipment remains essential. This chapter should enable researchers with little prior knowledge to set up a suitable laboratory to do basic cell culture, but there is still no substitute for experience within an existing well-run laboratory.
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References
Langdon, S. P. (2004) Basic principles of cancer cell culture, Methods Mol Med 88, 3–15.
Morgan, J. F., Morton, H. J., and Parker, R. C. (1950) Nutrition of animal cells in tissue culture; initial studies on a synthetic medium, Proc Soc Exp Biol Med 73, 1–8.
McCoy, T. A., Maxwell, M., and Kruse, P. F., Jr. (1959) Amino acid requirements of the Novikoff hepatoma in vitro, Proc Soc Exp Biol Med 100, 115–118.
Moore, G. E., and Pickren, J. W. (1967) Study of a virus-containing hematopoietic cell line and a melanoma cell line derived from a patient with a leukemoid reaction, Lab Invest 16, 882–891.
Moore, G. E., and Glick, J. L. (1967) Perspective of human cell culture, Surg Clin North Am 47, 1315–1324.
Bottenstein, J. E., and Sato, G. H. (1979) Growth of a rat neuroblastoma cell line in serum-free supplemented medium, Proc Natl Acad Sci USA 76, 514–517.
Sato, J. D., Cao, H. T., Kayada, Y., Cabot, M. C., Sato, G. H., Okamoto, T., and Welsh, C. J. (1988) Effects of proximate cholesterol precursors and steroid hormones on mouse myeloma growth in serum-free medium, In Vitro Cell Dev Biol 24, 1223–1228.
Ham, R. G. (1963) An improved nutrient solution for diploid Chinese hamster and human cell lines, Exp Cell Res 29, 515–526.
Bettger, W. J., and Ham, R. G. (1982) The nutrient requirements of cultured mammalian cells, Adv Nutr Res 4, 249–286.
Barnes, D., and Sato, G. (1979) Growth of a human mammary tumour cell line in a serum-free medium, Nature 281, 388–389.
Prasad, K. N., and Kumar, S. (1975) Role of cyclic AMP in differentiation of human neuroblastoma cells in culture, Cancer 36, 1338–1343.
Allegra, J. C., and Lippman, M. E. (1978) Growth of a human breast cancer cell line in serum-free hormone-supplemented medium, Cancer Res 38, 3823–3829.
Murakami, H., and Masui, H. (1980) Hormonal control of human colon carcinoma cell growth in serum-free medium, Proc Natl Acad Sci USA 77, 3464–3468.
Evan, G. I., Wyllie, A. H., Gilbert, C. S., Littlewood, T. D., Land, H., Brooks, M., Waters, C. M., Penn, L. Z., and Hancock, D. C. (1992) Induction of apoptosis in fibroblasts by c-myc protein, Cell 69, 119–128.
Harrington, E. A., Bennett, M. R., Fanidi, A., and Evan, G. I. (1994) c-Myc-induced apoptosis in fibroblasts is inhibited by specific cytokines, EMBO J 13, 3286–3295.
Fernando, A., Glaysher, S., Conroy, M., Pekalski, M., Smith, J., Knight, L. A., Di Nicolantonio, F., and Cree, I. A. (2006) Effect of culture conditions on the chemosensitivity of ovarian cancer cell lines, Anticancer Drugs 17, 913–919.
Heist, R. S., and Christiani, D. (2009) EGFR-targeted therapies in lung cancer: predictors of response and toxicity, Pharmacogenomics 10, 59–68.
Arena, S., Isella, C., Martini, M., de Marco, A., Medico, E., and Bardelli, A. (2007) Knock-in of oncogenic Kras does not transform mouse somatic cells but triggers a transcriptional response that classifies human cancers, Cancer Res 67, 8468–8476.
Balaguer, P., Boussioux, A. M., Demirpence, E., and Nicolas, J. C. (2001) Reporter cell lines are useful tools for monitoring biological activity of nuclear receptor ligands, Luminescence 16, 153–158.
Crook, T. J., Hall, I. S., Solomon, L. Z., Birch, B. R., and Cooper, A. J. (2000) A model of superficial bladder cancer using fluorescent tumour cells in an organ-culture system, BJU Int 86, 886–893.
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Cree, I.A. (2011). Principles of Cancer Cell Culture. In: Cree, I. (eds) Cancer Cell Culture. Methods in Molecular Biology, vol 731. Humana Press. https://doi.org/10.1007/978-1-61779-080-5_2
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DOI: https://doi.org/10.1007/978-1-61779-080-5_2
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