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Overview of the Generation, Validation, and Application of Phosphosite-Specific Antibodies

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Signal Transduction Immunohistochemistry

Part of the book series: Methods in Molecular Biology ((MIMB,volume 717))

Abstract

Protein phosphorylation is a universal key posttranslational modification that affects the activity and other properties of intracellular proteins. Phosphosite-specific antibodies can be produced as polyclonals or monoclonals in different animal species, and each approach offers its own benefits and disadvantages. The validation of phosphosite-specific antibodies requires multiple techniques and tactics to demonstrate their specificity. These antibodies can be used in arrays, flow cytometry, and imaging platforms. The specificity of phosphosite-specific antibodies is key for their use in proteomics and profiling of disease.

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Authors and Affiliations

  1. R&D Systems, Inc, Minneapolis, MN, USA

    Kathy Brumbaugh, Wade Johnson, Wen-Chieh Liao, Mong-Shang Lin, J. P. Houchins, Jeff Cooper, Steven Stoesz & Roberto Campos-Gonzalez

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  1. Kathy Brumbaugh
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  2. Wade Johnson
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  3. Wen-Chieh Liao
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  4. Mong-Shang Lin
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  5. J. P. Houchins
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  6. Jeff Cooper
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  8. Roberto Campos-Gonzalez
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Correspondence to Roberto Campos-Gonzalez .

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  1. R & D Systems, Inc., McKinley Place NE. 614, Minneapolis, 55413, Minnesota, USA

    Alexander E. Kalyuzhny

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Brumbaugh, K. et al. (2011). Overview of the Generation, Validation, and Application of Phosphosite-Specific Antibodies. In: Kalyuzhny, A. (eds) Signal Transduction Immunohistochemistry. Methods in Molecular Biology, vol 717. Humana Press. https://doi.org/10.1007/978-1-61779-024-9_1

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  • DOI: https://doi.org/10.1007/978-1-61779-024-9_1

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