Abstract
Allele-specific gene silencing by RNA interference (RNAi) is therapeutically useful for specifically inhibiting the expression of disease-associated alleles without suppressing the expression of corresponding wild-type alleles. To realize such allele-specific RNAi (ASP-RNAi), the design and assessment of small interfering RNA (siRNA) duplexes conferring ASP-RNAi is vital; however, it is also difficult. We have developed an assay system with reporter alleles that encode the Photinus and Renilla luciferase genes carrying mutant and wild-type allelic sequences in their 3′-untranslated regions. The assay system allows us to evaluate designed siRNAs and also short hairpin RNAs for allele-specific silencing against target mutant alleles as well as off-target silencing against corresponding wild-type alleles simultaneously in a qualitative and quantitative manner.
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Acknowledgments
The author would like to thank Yusuke Ohnishi and Mariko Yoshida for their helpful assistance. This work was supported by a research grant from the Ministry of Health, Labor, and Welfare of Japan and by a Grant-in-Aid from the Japan Society for the Promotion of Science.
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Hohjoh, H. (2010). Allele-Specific Silencing by RNA Interference. In: Min, WP., Ichim, T. (eds) RNA Interference. Methods in Molecular Biology, vol 623. Humana Press. https://doi.org/10.1007/978-1-60761-588-0_4
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DOI: https://doi.org/10.1007/978-1-60761-588-0_4
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