Abstract
Accumulated evidences indicate that reactive oxygen species (ROS) are involved in the pathophysiology of aging process. Antioxidants are believed to play an important role in the defense system to counteract ROS in the body. While excess hydrophilic antioxidants can be excreted easily in urine, lipophilic antioxidants can penetrate into blood lipoproteins and cell membranes, and may maintain long and high bioavailability. These lipophilic antioxidants are thus expected to contribute greatly to the prevention of age-related diseases.
Oils extracted from plant seeds are known to contain various lipophilic antioxidants such as vitamin E (α-tocopherol) and carotenoids. They are known to not only decrease serum low-density-lipoprotein (LDL) level, but also prevent oxidation of LDL. In addition to vitamin E (α-tocopherol) and carotenoids, other lipophilic antioxidants such as γ-oryzanol and sesaminol (from sesamolin) are in rice bran and sesame, respectively. They are sometimes called “vitamin-like food factors” or “biofactors.”
Although there are several methods for measuring the total antioxidant activities for various plant extracts, most of these methods are designed for hydrophilic antioxidants, and not for lipophilic antioxidants present in various plant seed oils.
In this report, we present an assay method for the total potency of antioxidants that are soluble in oil (PAO-SO) utilizing bathocuproine (BC) as a chromogen. BC-based antioxidant activity assay shows good linearity (r 2 = 0.9986), good reproducibility (CV < 10%), and good recovery (86–91%) when dl-α-tocopherol, for example, is added to sesame oil. Total antioxidant activity of rape-seed oil, olive oil, and sesame oil could also be successfully measured.
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References
Huang D, Ou B, Hampsch-Woodill M, Flanagan JA, Deemer EK (2002) Development and validation of oxygen radical absorbance capacity assay for lipophilic antioxidants using randomly methylated beta-cyclodextrin as the solubility enhancer. J Agric Food Chem 50:1815–1821
Chen L, Stacewicz-Sapuntzakis M, Duncan C, Sharifi R, Ghosh L, van Breemen R, Ashton D, Bowen PE (2001) Oxidative DNA damage in prostate cancer patients consuming tomato sauce-based entrees as a whole-food intervention. J Natl Cancer Inst 93:1872–1879
Gartner C, Stahl W, Sies H (1997) Lycopene is more bioavailable from tomato paste than from fresh tomatoes. Am J Clin Nutr 66:116–122
Vissers MN, Zock PL, Katan MB (2004) Bioavailability and antioxidant effects of olive oil phenols in humans: A review. Eur J Clin Nutr 58:955–965
Huang D, Ou B, Prior RL (2005) The chemistry behind antioxidant capacity assays. J Agric Food Chem 53:1841–1856
Apak R, Guclu K, Ozyurek M, Karademir SE (2004) Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. J Agric Food Chem 52:7970–7981
Apak R, Guclu K, Ozyurek M, Bektasoglu B, Bener M (2008) Cupric ion reducing antioxidant capacity assay for food antioxidants: vitamins, polyphenolics, and flavonoids in food extracts. In: Armstrong D (ed) Advanced protocols in oxidative stress I. Humana, NY, pp 163–193
Giuseppe DC (1998) Impiego della batocuproina (acido disolfonico) per la valutazione del potere antiossidante (PAO) nei liquidi e soluzioni. Italian patent 01309421
Giuseppe DC (1999) Use of bathocuproine for the evaluation of the antioxidant power in liquids and solutions. US patent 6,613,577
Martino M, Chiarelli F, Moriondo M, Torello M, Azzari C, Galli L (2001) Restored antioxidant capacity parallels the immunologic and virologic improvement in children with perinatal human immunodeficiency virus infection receiving highly active antiretroviral therapy. Clin Immunol 100:82–86
Preget P, Bollo E, Cannizzo FT, Biolatti B, Contato E, Biolatti PG (2005) Antioxidant capacity as a reliable marker of stress in dairy calves transported by road. Vet Rec 156:53–54
Falaschini A, Marangoni G, Rizzi S, Trombetta MF (2005) Effects of the daily administration of a rehydrating supplement to trotter horses. J Equine Sci 16:1–9
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Sakai, K., Kino, S., Takeuchi, M., Ochi, T., Da Cruz, G., Tomita, I. (2010). Analysis of Antioxidant Activities in Vegetable Oils and Fat Soluble Vitamins and Biofactors by the PAO-SO Method. In: Armstrong, D. (eds) Advanced Protocols in Oxidative Stress II. Methods in Molecular Biology, vol 594. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-411-1_16
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DOI: https://doi.org/10.1007/978-1-60761-411-1_16
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