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Lipidomic Analysis of Biological Samples by Liquid Chromatography Coupled to Mass Spectrometry

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Lipidomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 579))

Summary

Lipidomics studies the large-scale changes in nonwater-soluble metabolites (lipids) accompanying perturbations of biological systems. Because lipids are involved in crucial biological mechanisms, there is a growing scientific interest in using lipidomic approaches to understand the regulation of the lipid meta-bolism in all eukaryotic and prokaryotic organisms. Lipidomics is a powerful tool in system biology that can be used together with genomics, transcriptomics, and proteomics to answer biological questions arising from various scientific areas such as environmental sciences, pharmacology, nutrition, biophysics, cell biology, physiology, pathology, and disease diagnostics. One of the main challenges for lipidomic analysis is the range of concentrations and chemical complexity of different lipid species. In this chapter, we present a lipidomic approach that combines sample preparation, chromatographic, and intrasource ionization separation coupled to mass spectrometry for analyzing a broad-range of lipid molecules in biological samples.

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Acknowledgments

The contribution of the Agilent Technologies/University of California Irvine Analytical Discovery Facility, Center for Drug Discovery and the Agilent Technologies Foundation are gratefully acknowledged. This work was supported by grants from the National Institute of Health (R21DA-022702, R01DK-073955, R01 DA-012413, R01DA-012447, RR274–297/3504008, RR274–305/3505998, 1RL1AA017538 to D.P.).

Author information

Authors and Affiliations

  1. Department of Pharmacology, University of California – Irvine, Irvine, CA, USA

    Giuseppe Astarita & Daniele Piomelli

  2. Agilent Technologies, Irvine/Agilent Analytical Discovery Facility, University of California – Irvine, Irvine, CA, USA

    Faizy Ahmed

Authors
  1. Giuseppe Astarita
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  2. Faizy Ahmed
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  3. Daniele Piomelli
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Editors and Affiliations

  1. Golden Leaf Rd. 215, Mars Hill, 28754, U.S.A.

    Donald Armstrong

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Astarita, G., Ahmed, F., Piomelli, D. (2009). Lipidomic Analysis of Biological Samples by Liquid Chromatography Coupled to Mass Spectrometry. In: Armstrong, D. (eds) Lipidomics. Methods in Molecular Biology, vol 579. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-322-0_10

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  • DOI: https://doi.org/10.1007/978-1-60761-322-0_10

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-321-3

  • Online ISBN: 978-1-60761-322-0

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