Summary
Reflectometry is classified in comparison to the commercialized refractometric surface plasmon resonance (SPR). The advantages of direct optical detection depend on a sophisticated surface chemistry resulting in negligible nonspecific binding and high loading with recognition sites at the biopolymer sensitive layer of the transducer. Elaborate details on instrumental realization and surface chemistry are discussed for optimum application of reflectometric interference spectroscopy (RIfS). A standard protocol for a binding inhibition assay is given. It overcomes principal problems of any direct optical detection technique.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC, a part of Springer Science+Business Media, LLC
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Proll, G., Markovic, G., Steinle, L., Gauglitz, G. (2009). Reflectometric Interference Spectroscopy. In: Rasooly, A., Herold, K.E. (eds) Biosensors and Biodetection. Methods in Molecular Biology™, vol 503. Humana Press. https://doi.org/10.1007/978-1-60327-567-5_8
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DOI: https://doi.org/10.1007/978-1-60327-567-5_8
Publisher Name: Humana Press
Print ISBN: 978-1-60327-566-8
Online ISBN: 978-1-60327-567-5
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