Many sensors and biosensors are based on the detection of optical changes in the sensing phase. In order to build a stand-alone sensing device, a miniature and low-cost detection system is critical. Here, the method for manufacturing the most critical part (the photodetector) is described in detail. The receiver is based on a photodiode. The practical design of such device is presented here. By using it, it is possible to construct an optical sensor for fluorescence or absorption-based measurement. Discussed here are suitable methods for generation and modulation of probing light, as well as the possible optical configurations. Practical advice is given on the selection of the bandwidth, noise, amplification, etc. The described system was used for detection of green red fluorescent protein in E. coli during fermentation.
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Kostov, Y. (2009). Photodiode-Based Detection System for Biosensors. In: Rasooly, A., Herold, K.E. (eds) Biosensors and Biodetection. Methods in Molecular Biology™, vol 503. Humana Press. https://doi.org/10.1007/978-1-60327-567-5_17
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DOI: https://doi.org/10.1007/978-1-60327-567-5_17
Publisher Name: Humana Press
Print ISBN: 978-1-60327-566-8
Online ISBN: 978-1-60327-567-5
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