Summary
The use of Charge Coupled Device (CCD) detectors as an integral part of a biosensing system has become widespread in recent years due to several advantages of this type of detection, such as the ability to image multiple zones on the sensor, the flexibility of defining the sensing configuration and the low-noise performance of the detectors. The specification of the CCD as well as the selection of the other components in this system–including the source and the filters–is driven by the particular transduction mechanism, but all parts must be matched. Particular attention must be paid to reducing the various noise components of the CCD to obtain the lowest detection level, and it is shown that cooling the CCD is often a wise choice.
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References
Halasey, S. and Park, R. (Sept. 2002) Making sense of the global IVD market.IVD Technology, 29
See, for example, http://www.affymetrix.com
Rowe, C. A. et al. (1999) Array biosensor for simultaneous identification of bacterial, viral and protein analytes.Anal. Chem. 71, 3846–3852
Woo, J. and Henry, J. B. (1994) The advance of technology as a prelude to the laboratory of the twenty-first century.Clin. Lab. Med. 14, 459–471
Sapsford, K. E. et al. (2004) Fluorescence-based array biosensors for the detection of biohazards.J. Appl. Microbiol. 96, 47–58
Wolfbeis, O. S. (2002) Fiber-optic chemical sensors and biosensors.Anal. Chem. 74, 2663–2677
See, for example, http://www.astrovid.com
Howell, S. B. (2006)Handbook of CCD Astronomy, Second Edition. Cambridge University Press, Cambridge
Herron, J. N. et al. (1998) Rapid clinical diagnostics arrays using injection-molded planar waveguides.SPIE 3259, 54–64
Tolley, S. et al. (2003) Single-chain polymorphism analysis of long QT syndrome using planar waveguide fluorescent biosensors.Anal. Biochem. 315, 223–237
Herron, J. N. et al. (Oct. 14, 1997) Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays. US Patent No. 5,677,196
Males, R. G. et al. (2001) Cardiac markers and point-of-care testing: a perfect fit.Crit. Care Nurs. 24, 54–61
Fivash, M. et al. (1998) BIAcore for macro-molecular interaction.Curr. Opin. Biotech-nol. 9, 97–101
Zhang, X. et al. (2005) Surface-enhanced Raman spectroscopy biosensors: excitation spectroscopy for optimization of substrates fabricated by nanosphere lithography.IEEE Proc. Nanobiotechnol. 152, 195–206
Williams, C. S. and Becklund, O. A. (1972)Optics: A Short Course for Engineers and Scientists. Wiley-Interscience, New York. Chap. 6
Litwiller, D. (Jan. 2001)CCD vs. CMOS: facts and fiction. Photonics Spectra, 154–158
Agrawal, G. P. (1992)Fiber-Optic Communications Systems. Wiley-Interscience, New York. Chap. 4
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Christensen, D.A., Herron, J.N. (2009). Optical System Design for Biosensors Based on CCD Detection. In: Rasooly, A., Herold, K.E. (eds) Biosensors and Biodetection. Methods in Molecular Biology™, vol 503. Humana Press. https://doi.org/10.1007/978-1-60327-567-5_13
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DOI: https://doi.org/10.1007/978-1-60327-567-5_13
Publisher Name: Humana Press
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