Summary
Cytogenetics is integral to the diagnosis of childhood leukaemia, particularly in relation to the risk stratification of patients for treatment. Fluorescence in situ hybridization (FISH) has become an important complementary technique, expanding chromosomal analysis into the molecular arena. It has greatly improved the accuracy and applicability of cytogenetics and led to the discovery of novel chromosomal changes of prognostic significance. Many probes are now commercially available, providing robust and reliable detection of chromosomal abnormalities. Since the cloning of the human genome, it is now possible to access detailed genomic information and develop FISH probes for virtually any known DNA sequence. The range of procedures necessary for the successful application of FISH in the accurate detection of significant chromosomal abnormalities in childhood acute leukaemia is described here.
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References
Grimwade D, Walker H, Oliver F, Wheatley K, Harrison CJ, Harrison G, et al. (1998). The importance of diagnostic cytogenetics on outcome in AML: Analysis of 1,612 patients entered into the MRC AML 10 trial. Blood 92(7):2322–33.
Harrison CJ, Foroni L. (2002). Cytogenetics and molecular genetics of acute lymphoblastic leukemia. Rev Clin Exp Hematol;6(2):91–113.
Romana SP, Poirel H, Leconiat M, Flexor MA, Mauchauffe M, Jonveaux P, et al. (1995). High frequency of t(12;21) in childhood B-lineage acute lymphoblastic leukemia. Blood;86:4263–9.
Robinson HM, Martineau M, Harris RL, Barber KE, Jalali GR, Moorman AV, et al. (2005). Derivative chromosome 9 deletions are a significant feature of childhood Philadelphia chromosome positive acute lymphoblastic leukaemia. Leukemia;19(4):564–71.
Pinson MP, Martineau M, Jabbar MS, Kilby AM, Walker H, Harrison CJ. (2000). Sequential FISH reveals an abnormal karyotype involving 14 chromosomes in a child with acute lymphoblastic leukemia. Leukemia;14(9):1705–7.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Konn, Z.J., Wright, S.L., Barber, K.E., Harrison, C.J. (2009). Fluorescence In Situ Hybridization (FISH) as a Tool for the Detection of Significant Chromosomal Abnormalities in Childhood Leukaemia. In: Eric So, C.W. (eds) Leukemia. Methods in Molecular Biology™, vol 538. Humana Press. https://doi.org/10.1007/978-1-59745-418-6_3
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DOI: https://doi.org/10.1007/978-1-59745-418-6_3
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